Project description:To understand the role of LSD1 in transcriptional regulation in muscle under voluntary wheel running (VWR) training, RNA-seq analyses of soleus muscles of skeletal muscle-specific LSD1 KO mice (LSD1-mKO mice) and WT mice after VWR were carried out. We found that loss of LSD1 led to increased expression of oxidative metabolism genes in soleus muscle.

2023-01-24 | GSE198827 | GEO

Analysis of LPS stimulation on F480low CD11b High Macrophages from WT and muMT KO mice

Project description:Single cell suspensions from WT or muMT KO mice were stimulated with LPS for 2 hours after which macrophages were sorted

2022-11-01 | GSE139854 | GEO

TTP-dependent mRNA decay in LPS-stimulated macrophages

2011-12-15 | GSE28880 | GEO

Analysis of LPS stimulation on F480Hi CD11blo Macrophages from WT and PI3KδE1020K-B KI mice

Project description:Single cell suspensions from WT or PI3KδE1020K-B KI mice were stimulated with LPS for 2 hours after which macrophages were sorted

2022-11-01 | GSE139852 | GEO

TTP-dependent mRNA decay in LPS-stimulated macrophages

Project description:Controlled decay of cytokine and chemokine mRNAs restrains the time and amplitude of inflammatory responses. Tristetraprolin (TTP) binds to AU-rich elements in 3´ untranslated regions of mRNA and targets the bound mRNA for degradation. We have addressed here the function of TTP in balancing the macrophage activation state by a comprehensive analysis of TTP-dependent mRNA decay in LPS-stimulated macrophages from WT and TTP-deficient mice. We compared mRNA stability in LPS-treated BMDMs from WT and TTP-/- mice by microarray-based measurement of the remnant mRNA after transcriptional blockade with actinomycin D (act D). To increase the sensitivity of the mRNA decay profiling we inhibited the LPS-activated p38 MAPK with the specific inhibitor SB203580 since p38 MAPK negatively regulates the mRNA-destabilizing activity of TTP. LPS stimulation was for 3h before addition of act D. RNA was harvested at 0', 45' and 90' thereafter.

2011-12-15 | E-GEOD-28880 | biostudies-arrayexpress

Analysis of LPS stimulation on F480Low CD11b High Macrophages from WT and PI3KδE1020K-B KI mice

Project description:Single cell suspensions from WT or PI3KδE1020K-B KImice were stimulated with LPS for 2 hours after which macrophages were sorted

2022-11-01 | GSE139855 | GEO

Sequencing of LPS-stimulated immune cells from chicken

Project description:RNAs were analysed from bone marrow derived dendritic cells from 6 week old birds (control and LPS stimulated), bone marrow derived macrophages from 6 week old birds (control and LPS stimulated) and heterophils isolated from blood of day-old chicks (control and LPS stimulated).

2015-01-19 | E-MTAB-2996 | biostudies-arrayexpress

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