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Drosophila melanogaster

ABSTRACT: Expression data from eye antennal imaginal disc expressing either activated Ras or secreted Spitz

OTHER RELATED OMICS DATASETS IN: E-GEOD-6300

PROVIDER: PRJNA100499 | ENA |

REPOSITORIES: ENA

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Transcription profiling of Drosophila eye antennal imaginal disc expressing either activated Ras or secreted Spitz

Project description:We have used microarrays to identify genes expressed and required for the second mitotic wave (SMW) during eye development. Eye discs expressing Spitz under the control of GMR Gal4 have no SMW as Spitz promotes G1 arrest, ectopic differentiation also occures. To control for the ectopic differentiation, Spi expressing eye antennal discs were compared to eye antennal discs expressing activated RasV12. In discs expresseding RasV12 under the control of GMRGal4 the SMW takes place normally prior to any ectopic differentiation. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Experiment Overall Design: Drosophila eye antennal imaginal discs expressing either UAS RasV12 or UAS Spi under the control of GMRGal4 were dissected from 3rd instar larvae for RNA extraction and hybridization on Affymetrix microarrays.

2008-06-14 | E-GEOD-6300 | biostudies-arrayexpress

Expression data from eye antennal imaginal disc expressing either activated Ras or secreted Spitz

Project description:We have used microarrays to identify genes expressed and required for the second mitotic wave (SMW) during eye development. Eye discs expressing Spitz under the control of GMR Gal4 have no SMW as Spitz promotes G1 arrest, ectopic differentiation also occures. To control for the ectopic differentiation, Spi expressing eye antennal discs were compared to eye antennal discs expressing activated RasV12. In discs expresseding RasV12 under the control of GMRGal4 the SMW takes place normally prior to any ectopic differentiation. We used microarrays to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Keywords: Comparison of eye antennal discs of two different over expression genotypes

2007-11-14 | GSE6300 | GEO

RNA-seq in wild-type and glass mutant eye-antennal discs in Drosophila melanogaster

Project description:The aim of this data set is to perform a differential expression analysis between wild type eye-antennal imaginal disc and discs that are homozygous glass mutant gl[60j]. This data set is used to validate Glass target gene predictions identified by i-cisTarget on a set of conserved eye-specific genes. RNA-seq was performed in eye-antennal imaginal discs of two D.melanogaster wild-type strains (Canton S and strain RAL-208 (Jordan et al. 2007, Ayroles et al. 2009)), representing two biological replicates; and in glass mutant (gl[60j]) discs for two technical replicates.

2012-09-21 | E-GEOD-39781 | biostudies-arrayexpress

RNA-seq in wild-type and glass mutant eye-antennal discs in Drosophila melanogaster

2012-09-21 | GSE39781 | GEO

Hippo signaling instructs ectopic but not normal organ growth [Drosophila YkiHyper]

Project description:In order to study how ectopic Yki drives tissue overgrowth in Drosophila imaginal discs, we overexpressed the constitutively active Yki3SA and deleted wts in clones of cells in the entire eye-antennal imaginal disc, as well as specifically in eye disc proper cells using Optix-Gal4. Using the MARCM system allowed us to compare the effects of Yki3SA overexpression in wild-type and sd mutant clones.

2022-11-19 | GSE211458 | GEO

Drosophila melanogaster

Project description:Eye imaginal disc development

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Drosophila melanogaster

Project description:Expression data from transdetermined Drosophila eye antennal imaginal disc by winged eye overexpression.

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Project description:Toy in the eye-antennal imaginal disc

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Analysis of Drosophila melanogaster Chromosome Topology in Larval Eye and Antennal Discs

Project description:The goal of this study was to examine chromosome topology in Drosophila larval eye and antennal discs by identifying topologically associating domains (TADs) across the genome. TADs were compared between the eye and antennal disc to determine whether they contribute to cell-type-specific homologous pairing and transvection.

2019-08-24 | GSE136267 | GEO

D. melanogaster eye-antennal imaginal discs developmental transcriptome

Project description:Transcriptomes of Drosophila melanogaster eye-antennal imaginal discs at three sequential larval stages: late 2nd instar (72h after egg laying (AEL)), mid 3rd instar (96h AEL) and late 3rd instar (120h AEL).

2018-01-08 | GSE94915 | GEO

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