Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNAseq of SMAD2/3DKO and Smad4KO in pluripotent mESC and EBs

ABSTRACT: RNAseq of SMAD2/3DKO and Smad4KO in pluripotent mESC and EBs

PROVIDER: PRJNA1005247 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25630031_1.fastq.gz	Fastqsanger.gz
	SRR25630031_2.fastq.gz	Fastqsanger.gz
	SRR25630032_1.fastq.gz	Fastqsanger.gz
	SRR25630032_2.fastq.gz	Fastqsanger.gz
	SRR25630033_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

RNAseq & Smad and Foxh1 ChIpseq in pluripotent mESC and EBs

Project description:Smad2, Smad3, Smad4 and Foxh1 ChIPseq performed in pluripotent mESC and embryonic bodies (EBs). RNAseq were performed in WT mESCs and Ebs of WT, Smad2KO, Smad3KO and Smad2/3DKO.

2019-09-05 | GSE125116 | GEO

RNAseq of SMAD2/3DKO and Smad4KO in pluripotent mESC and EBs

Project description:To dissected the roles of SMAD2/3 and SMAD4 in mouse embryonic stem cells (mESC), knockouts of SMAD2/3 (SMAD2 and SMAD3 double knockout, S2/3DKO) and SMAD4 (SMAD4 knockout, S4KO) were used to conduct RNAseq in ES and EB conditions.

2024-10-21 | GSE240785 | GEO

RNAseq & Smad and Foxh1 ChIpseq in pluripotent mESC and EBs

Project description:RNAseq & Smad and Foxh1 ChIpseq in pluripotent mESC and EBs

| PRJNA515268 | ENA

The binding sites of SMAD2/3, Dnmt3a and Dnmt3b in pluripotent mESC, EBs and ME when Smad4 is lost with or without Dnmt3b knockdown

Project description:To understand the genome wide binding profile of SMAD2/3 when SMAD4 is lost, Smad2/3 ChIPseq was performed in SMAD4 knockout (S4KO) mouse embryonic stem cells (mESC) or in S4KO embryonic bodies (EBs). To figure out the effect of Dnmt3b on SMAD2/3 binding ability in the absence of SMAD4, we further performed SMAD2/3 CUT&Tag in EBs of S4KO and S4KO with Dnmt3b konckdown, as well as in mesendoderm (ME) stage of WT and S4KO. The binding sites of Dnmt3a and Dnmt3b were explored by Dnmt3a or Dnmt3b ChIP-seq and Dnmt3b CUT&Tag in WT EBs and S4KO EBs. WT with Dnmt3b knockdown and S4KO with Dnmt3b knockdown were used to as a control for the Dnmt3b CUT&Tag. Besides, we inserted a 3xFlag-tag at the C-terminus of Dnmt3a or Dnmt3b and then carried out Flag CUT&Tag in EBs.

2024-10-21 | GSE240786 | GEO

The binding sites of SMAD2/3, Dnmt3a and Dnmt3b in pluripotent mESC, EBs and ME when Smad4 is lost with or without Dnmt3b knockdown

Project description:The binding sites of SMAD2/3, Dnmt3a and Dnmt3b in pluripotent mESC, EBs and ME when Smad4 is lost with or without Dnmt3b knockdown

| PRJNA1005248 | ENA

SMAD2/3 interactome in human pluripotent stem cells

Project description:This experiment was designed to determine the interactome of SMAD2/3 in human pluripotent stem cells (hPSCs). hPSCs were cultured in standard pluripotency-promoting conditions, or induced to differentiate towards the definitive endoderm lineage for 36h. Endogenous SMAD2/3 was immunoprecipitated from nuclear extracts in these two conditions using a specific antibody. Non-immune IgG immunoprecipitations were performed as negative controls. Three biological replicates per conditions were analyzed by quantitative label-free mass spectrometry.

2018-01-04 | PXD005285 | Pride

RNAseq of setd2KO mESC

Project description:RNAseq of setd2KO mESC

| PRJEB67474 | ENA

K562+mESC bulk RNAseq

Project description:bulk RNAseq of K562 and mESC

| PRJEB67943 | ENA

RNAseq in protected BAP1 mESc

Project description:RNAseq in protected BAP1 mESc

| PRJNA493016 | ENA

Bulk RNAseq REX-1 RFP mESC 2019

Project description:Bulk RNAseq REX-1 RFP mESC 2019