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Transcriptional analysis of transposon mutants disrupted in glutathione or ergothioneine biosynthesis in Burkholderia thailandensis

ABSTRACT: Transcriptional analysis of transposon mutants disrupted in glutathione or ergothioneine biosynthesis in Burkholderia thailandensis

PROVIDER: PRJNA1005314 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25635600_1.fastq.gz	Fastqsanger.gz
	SRR25635600_2.fastq.gz	Fastqsanger.gz
	SRR25635601_1.fastq.gz	Fastqsanger.gz
	SRR25635601_2.fastq.gz	Fastqsanger.gz
	SRR25635602_1.fastq.gz	Fastqsanger.gz

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Transcriptional analysis of transposon mutants disrupted in glutathione or ergothioneine biosynthesis in Burkholderia thailandensis

Project description:In this study we compare logarithmically grown Burkholderia thailadensis wildtype to a transposon mutant that is disrupted in gshA1, or gshA2, which are involved in glutathione biosynthesis or egtB, involved in ergothioneine biosynthesis using RNASeq to identify novel pathways where glutathione or ergothioneine may be involved.

2024-08-22 | GSE240826 | GEO

Transcriptional analysis of transposon mutants disrupted in glutathione biosynthesis in Acinetobacter baumannii

Project description:In this study we compare logrithmically grown Acinetobacter baumannii wildtype to a transposon mutant that is disrupted in gshA, the first step in glutathione biosynthesis using RNASeq to identify novel pathways where glutathione may be involved.

2022-01-08 | GSE192957 | GEO

Transcriptional analysis of transposon mutants disrupted in glutathione biosynthesis in Pseudomonas aeruginosa

Project description:In this study we compare logarithmically grown Pseudomonas aeruginosa wildtype to a transposon mutant that is disrupted in gshA, which is involved in glutathione biosynthesis using RNASeq to identify novel pathways where glutathione may be involved.

2024-08-22 | GSE240828 | GEO

Transcriptional analysis of transposon mutants disrupted in glutathione biosynthesis in Pseudomonas aeruginosa

Project description:Transcriptional analysis of transposon mutants disrupted in glutathione biosynthesis in Pseudomonas aeruginosa

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Transcriptional analysis of transposon mutants disrupted in glutathione biosynthesis in Acinetobacter baumannii

Project description:Transcriptional analysis of transposon mutants disrupted in glutathione biosynthesis in Acinetobacter baumannii

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Transcriptional analysis of transposon mutants disrupted in bacillithiol biosynthesis in Staphylococcus aureus

Project description:In this study we compare logrithmically grown Staphylococcus aureus SAUSA300 wildtype to a transposon mutant that is disrupted in bshC, the third step in bacillithiol biosynthesis using RNASeq to identify novel pathways where bacillithiol may be involved.

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Transcriptional analysis of transposon mutants disrupted in bacillithiol biosynthesis in Staphylococcus aureus

Project description:Transcriptional analysis of transposon mutants disrupted in bacillithiol biosynthesis in Staphylococcus aureus

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Genotyping of S. aureus SH1000 transposon mutants to identify mutant positions

Project description:A library of transposon mutants were generated in S.aureus strain SH1000 using a custom Mariner transposon with an outward-facing T7 promoter. Genomic DNA was extracted from the library and digested with an appropriate restriction enzyme (AluI or RsaI). Labelled RNA run-offs were produced from the T7 promoter and hybridised to a tiling microarray to determine the position of the mutant. This was done using a library of a million S. aureus mutants, and genes not disrupted by a transposon were inferred to be essential for replication and growth in vitro.

2009-04-16 | E-MEXP-1588 | biostudies-arrayexpress

B. thailandensis genes induced by subinhibitory concentration of glyphosate

Project description:Total transcript amplification (TTA) from single eukaryotic cells for transcriptome analysis is established, but TTA from a single prokaryotic cell presents additional challenges with much less starting material and lack of poly(A)-tails. We described, here, a novel method for single bacterium TTA, using a Burkholderia thailandensis model exposed to subinhibitory concentration of the antibacterial agent, glyphosate. Utilizing B. thialandensis microarray to assess the TTA method showed little gene bias (< 2 fold-change) and absence (~5-6%) when compared to the larger scale non-amplified RNA samples. B. thailandensis genes important to possibly recuperate and balance the intracellular amino acid pool were induced (or repressed) by the aromatic amino acid biosynthesis inhibitor, glyphosate. We validated our single-cell microarray data at the multi-cells and single-cell levels with lacZ and gfp reporter-gene fusions, respectively. This novel method will rejuvenate and expand the prokaryotic transcriptomic field. Two identical cultures of B. thailandensis wildtype strain E264 were grown in 1x M9 minimal medium supplemented with 1% Brij-58 and 20 mM glucose (MG) to mid-log phase. One culture was induced by final concentration of 0.01% (w/v) glyphosate for 30 minutes. Total RNA was then purified from B. thailandensis uninduced and induced samples, converted to cDNA and hybridized onto B. thailandensis 70mer triplicate array

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Regulation of Choline Catabolism in Burkholderia thailandensis

Project description:Burkholderia thailandensis is a soil-dwelling bacterium that shares many metabolic pathways with the ecologically similar, but evolutionarily distant, Pseudomonas aeruginosa. Among the diverse nutrients it can utilize is choline, which can be converted into the osmoprotectant glycine betaine and further catabolized as a source of carbon and nitrogen, similar to P. aeruginosa. Orthologs of genes in the choline catabolic pathway in these two bacteria showed distinct differences in gene arrangement as well as an additional orthologous transcriptional regulator in B. thailandensis. In this study, we showed that multiple glutamine amidotransferase1 (GATase1)-containing AraC-family transcription regulators (GATRs) are involved in regulation of the B. thailandensis choline catabolic pathway (gbdR1, gbdR2, souR). Using genetic analyses and sequencing the transcriptome in the presence and absence of choline, we identified the likely regulons of gbdR1 (BTH_II1869) and gbdR2 (BTH_II0968). We also identified a functional ortholog for P. aeruginosa souR, a GATR that regulates the metabolism of sarcosine to glycine. GbdR1 is absolutely required for expression of the choline catabolic locus, similar to P. aeruginosa GbdR, while GbdR2 is important to increase expression of the catabolic locus. Additionally, the B. thailandensis SouR ortholog (BTH_II0994) is required for catabolism of choline and its metabolites as carbon sources, whereas in P. aeruginosa, SouR function can by bypassed by GbdR. The strategy employed by B. thailandensis represents a distinct regulatory solution to control choline catabolism and thus provides both an evolutionary counterpoint and an experimental system to compare the acquisition and regulation of this pathway during environmental growth and infection.

2016-07-12 | GSE81652 | GEO

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