Project description:Transcriptomic changes in the crown (stem base) of barley (Hordeum vulgare L. cv. Golden Promise) during crown-root initiation and development
Project description:NILs containing five parental lines, three wild barley genotypes ssp. spontaneum: HID 4 (A), Iraq; HID 64 (B), Turkey; and HID 369 (C), Israel, one ssp. agriocrithon: HID 382(D)) and cv. Morex (ssp. vulgare, USA). Purpose: Variant calling to identifie markers associated with a awn length QTL on the distal part of chromosome 7HL
Project description:Wild-type barley cv Golden Promise and two independent RNAi knockdown lines (W1-1 And W1-7) of the WHIRLY1 gene were grown under controlled environments. Leaves were harvested after 7 or 14 days and sampled along a developmental gradient of base, mid and tip. Microarray analysis was conducted from three biological replicates using a custom designed Agilent barley microarray. Analysis of transcript abundance suggests developmental delay in WHIRLY knockdown lines with significant disruption of plastid development and transcription.
Project description:RNA sequencing of the near-isogenic line mnd1.a in cv. Bowman background, compared to cv. Mesa and cv. Bowman Purpose: Expression analysis and variant calling.
