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Developmental Transcription Profiles of Wild-Type and WHIRLY Knockdown Barley Leaves

ABSTRACT: Wild-type barley cv Golden Promise and two independent RNAi knockdown lines (W1-1 And W1-7) of the WHIRLY1 gene were grown under controlled environments. Leaves were harvested after 7 or 14 days and sampled along a developmental gradient of base, mid and tip. Microarray analysis was conducted from three biological replicates using a custom designed Agilent barley microarray. Analysis of transcript abundance suggests developmental delay in WHIRLY knockdown lines with significant disruption of plastid development and transcription.

INSTRUMENT(S): Agilent G2505B

ORGANISM(S): Hordeum vulgare

SUBMITTER: Pete Hedley

PROVIDER: E-MTAB-9882 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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