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ABSTRACT: Listeria monocytogenes GshF-regulated transcriptomics under normal conditions

PROVIDER: PRJNA1006145 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25659515_1.fastq.gz	Fastqsanger.gz
	SRR25659515_2.fastq.gz	Fastqsanger.gz
	SRR25659516_1.fastq.gz	Fastqsanger.gz
	SRR25659516_2.fastq.gz	Fastqsanger.gz
	SRR25659517_1.fastq.gz	Fastqsanger.gz

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Listeria monocytogenes SOS response determined after mitomycin C exposure

Project description:The SOS response is a conserved pathway that is activated under certain stress conditions and is regulated by the repressor LexA and the activator RecA. The food-borne pathogen Listeria monocytogenes contains RecA and LexA homologs, but their roles in Listeria have not been established. In this study, we identified the SOS regulon in L. monocytogenes by comparing the transcription profiles of the wild-type strain and the ΔrecA mutant strain after exposure to the DNA damaging agent mitomycinC (MMC). The SOS response is an inducible pathway involved in DNA repair, restart of stalled replication forks, and in induction of genetic variation in stressed and stationary phase cells. It is regulated by LexA and RecA. LexA is an autoregulatory repressor which binds to a consensus sequence in the promoter region of the SOS response genes, thereby repressing transcription. A consensus LexA binding motif for L. monocytogenes has not been identified thus far. Generally, the SOS response is induced under circumstances in which single stranded DNA accumulates in the cell. This results in activation of RecA, which in turn stimulates cleavage of LexA, and ultimately in the induction of the SOS response. Keywords: stress response, loop design, SOS response, mitomycin c, listeria monocytogenes, RecA, LexA

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Listeria monocytogenes J0161 shows altered resistance characteristics when grown at low temperature and under anaerobic conditions.

Project description:Listeria monocytogenes is a food-borne pathogen and the causative agent of listeriosis, an infection which typically arises through the consumption of contaminated foodstuffs. L. monocytogenes is a psychotrophic and facultatively anaerobic; properties which permit growth under refrigeration conditions and within modified atmosphere packaging. Through transcriptional changes L. monocytogenes is able to mount adaptive responses against stressors. Such responses typically cross protect against subsequent stresses.

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Gene expression profiling of a pressure-tolerant Listeria monocytogenes Scott A ctsR deletion mutant

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Gene expression profiling of a nisin-sensitive Listeria monocytogenes Scott A ctsR deletion mutant

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CtsR Regulon in Listeria monocytogenes

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