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ABSTRACT: Mismatch target library for VEGFA (T2)

PROVIDER: PRJNA1006348 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25663124.fastq.gz	Fastqsanger.gz
	SRR25663125.fastq.gz	Fastqsanger.gz
	SRR25663126.fastq.gz	Fastqsanger.gz

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Project description:1 Mismatch target library for VEGFA (T2)

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Project description:In ischemic stroke, DWI-T2 mismatch (positive signals on DWI but negative signals on T2) indicates ischemia within 4.5 h. However, the molecular expression pattern of this region remains elusive. This project aimed to reveal the proteomics profiling of the brain tissues at DWI-T2 mismatch, T2(+), and contralateral regions of brain within 4.5 h after middle cerebral artery occlusion compared with naïve brains of mice.

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Project description:CIRCLE-seq of VEGFA (T2)

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Nucleosome sliding by the Chd1 chromatin remodeler relies on the integrity of the DNA duplex

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Project description:We have characterized gene expression changes in HeLa cells following long term depletion of Cyclin T2 or Cyclin T1 using shRNA HeLa cells were transduced with VSV-G pseudotyped lentiviral vectors expressing shRNA against either Cyclin T2 or Cyclin T1. As a control, cells were transduced with shRNA vector with four nucleotides mismatch in the Cyclin T1 mRNA that has been previously shown to have minimal effects on mRNA expression levels. The vectors have a GFP reporter that can be used to estimate transduction efficiency. Five days post-transduction, cells were harvested and total RNA extracted. Transcriptional profiling was carried out on these RNA samples. Two independent biological replicate experiments were carried out in this analysis and the xpression values normalized by GC-RMA and averaged. The following comparisons were made: MM to Cyclin T2 and MM to Cyclin T1.

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