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Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [GRO-seq]

ABSTRACT: Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [GRO-seq]

PROVIDER: PRJNA1007637 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25723300_1.fastq.gz	Fastqsanger.gz
	SRR25723300_2.fastq.gz	Fastqsanger.gz
	SRR25723301_1.fastq.gz	Fastqsanger.gz
	SRR25723301_2.fastq.gz	Fastqsanger.gz
	SRR25723302_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [GRO-seq]

Project description:The ZFX transcriptional activator binds to CpG island promoters, with a major peak at ~200-250bp downstream from transcription start sites. Because ZFX binds within the transcribed region, we investigated whether it regulates transcriptional elongation. We used GRO-seq to show that loss or reduction of ZFX increased the pause ratio at ZFX-regulated promoters. To further investigate the mechanisms by which ZFX regulates transcription, we determined regions of the protein needed for transactivation and for recruitment to the chromatin. Interestingly, although ZFX has 13 grouped zinc fingers, deletion of the first 11 fingers produces a protein that can still bind to chromatin and activate transcription. We next used TurboID-MS to detect ZFX-interacting proteins, identifying ZNF593, proteins that interact with the N-terminal transactivation domain (e.g. histone modifying proteins), and proteins that interact with ZFX when it is bound to the chromatin (e.g. TAFs and other histone modifying proteins). Our studies support a model in which ZFX enhances elongation at target promoters by recruiting H4 acetylation complexes and reducing pausing.

2024-04-23 | GSE241281 | GEO

Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [RNA-Seq]

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Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [ChIP-Seq]

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Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters

Project description:Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters

| PRJNA1007628 | ENA

Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [ChIP-Seq]

Project description:Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [ChIP-Seq]

| PRJNA1007617 | ENA

Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [RNA-Seq]

Project description:Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters [RNA-Seq]

| PRJNA1007634 | ENA

Reduction of ZFX levels decreases histone H4 acetylation and increases the pause ratio at target promoters

2024-08-09 | PXD044709 | Pride

Reduction of ZFX levels decreases histone H4 acetylation and increases Pol2 pausing at target promoters

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-23 | GSE241284 | GEO

ZFX silencing introduced differential gene expression in leukemia cells

Project description:Zinc finger protein, X-linked (ZFX) is deregulated in various human leukemia patients. The silence of ZFX leads to the inhibited growth and enhance drug sensitivity of both K562 cells and Jurkat cells. To obtain molecular insights of how ZFX controls the proliferation and drug sensitivity of these cells, we generate the microarray data comparing control and ZFX silenced K562 and Jurkat cells.

2014-06-30 | GSE57552 | GEO

Zfx controls self-renewal of human embryonic stem cells

Project description:Previously, we identified the transcription factor Zfx as a key regulator of self-renewal in murine ESCs. Here we extend those findings to human ESCs. Zfx knockdown in hESCs hindered clonal growth and decreased colony size after serial replating. Zfx overexpression enhanced clone formation, increased colony size and decreased expression of differentiation-related genes in human ESCs. Zfx-overexpressing hESCs resisted spontaneous differentiation but could be directed to differentiate into endodermal and neural cell fates when provided with the appropriate cues. Thus, Zfx acts as a molecular rheostat regulating the balance between self-renewal and differentiation in hESCs, revealing the close evolutionary conservation of the self-renewal mechanisms in murine and human ESCs. Human embryonic stem cells with over expression of ZFX were compared to the original cell line (H9) and a clonally derived wild-type cell line (clone 3)

2012-01-01 | E-GEOD-29784 | biostudies-arrayexpress

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