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Whole-genome bisulfite sequencing of Infectious spleen and kidney necrosis virus

ABSTRACT: Whole-genome bisulfite sequencing of Infectious spleen and kidney necrosis virus

PROVIDER: PRJNA1008356 | ENA |

REPOSITORIES: ENA

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Whole-genome bisulfite sequencing of Infectious spleen and kidney necrosis virus

Project description:To obtain the site-by-site methylation landscape of the infectious spleen and kidney necrosis virus (ISKNV) genome, whole-genome bisulfite sequencing (WGBS) was performed on an ISKNV strain from 3 duplicate samples.

2023-08-23 | GSE241500 | GEO

Infectious spleen and kidney necrosis virus

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Transcription profiling by array of Atlantic salmon head kidney cells infected with infectious pancreatic necrosis virus

Project description:Infectious pancreatic necrosis (IPN) is a serious viral disease that causes significant economic losses in salmon aquaculture. To characterize the host-pathogen relationship in IPN, we analysed transcriptional profiles of salmon head kidney (SHK-1) cells infected with infectious pancreatic necrosis virus (IPNV) at three timepoints over six days (at 1, 3 & 6 days post infection. The transcriptome was investigated using the TRAITS / SGP 16950-feature Atlantic salmon cDNA microarray, which is enriched for genes with functions related to the immune response.

2011-01-01 | E-TABM-1000 | biostudies-arrayexpress

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Gene expression profile after oral vaccination against infectious pancreatic necrosis virus (IPNV)

2012-07-19 | GSE31591 | GEO

Gene expression profile after oral vaccination against infectious pancreatic necrosis virus (IPNV)

Project description:Oral immunization of trout against infectious pancreatic necrosis virus (IPNV) have been recently reported by using a DNA vector coding the viral capsid VP2 gene encapsulated in alginate microspheres. We report here an study of the transcripts in head kidney and pyloric ceca 7 days after oral vaccination of rainbow trout by using a newly designed rainbow trout 60-mer oligo microarray focused in their immune-related genes. Trout were obtained from 4 different farms, one group of trout was defined as belonging to one of the farms. Each of the trout 4 groups were divided into two subgroups of 6 trout for each subgroup. Subgroup I was orally vaccinated with 10 M-BM-5l of suspension of the vaccine microspheres each containing 10 M-BM-5g of pcDNA-VP2 diluted in 10M-BM-5l of PBS, while subgroup II received similar microspheres suspension but pcDNA. Head kidney and pyloric ceca were collected from each subgroup and RNA was extracted.

2012-07-18 | E-GEOD-31591 | biostudies-arrayexpress

Mixing Experiment - Mouse Kidney and Spleen

Project description:The design of this experiment was based on the assumption that transcript levels will change linearly while going from 100 percent of one tissue to 100 percent of the other. This assumed linear data set could be used to evaluate various issues related to low-level microarray data analysis. Hybridization cocktails from two mouse tissues, kidney and spleen, were prepared and mixed in a range of ratios and applied to 4-5 replicate GLYCOv1 chips for analysis. The mixture ratios were as follows: 100 percent kidney, 75 percent Kidney/25 percent spleen, 50 percent kidney/50 percent spleen, 25 percent kidney/75 percent spleen, and 100 percent spleen.

2011-03-02 | E-GEOD-27587 | biostudies-arrayexpress

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