Project description:Rhinoceromics -Relation between microbiome, mycobiome and diet composition on TS% levels in captive black rhinoceros (Diceros bicornis michaeli)
Project description:Small molecule analysis of Rhino serum extracts from Diceros bicornis, Rhinoceros unicornis, and Ceratotherium simum simum. Standard methanol extraction. Data were acquired using a Bruker Impact Maxis MS and C18 RP-UHPLC. Positive polarity acquisition of LC-MS/MS.
Project description:Efforts to implement effective assisted reproductive technologies (ART) to extricate the northern white rhinoceros (NWR; Ceratotherium simum cottoni) from extinction, could be unconventionally offset from studies carried out using the southern white rhinoceros (Ceratotherium simum simum) as a relative model species. The bi-directional communication and critical transport of regulatory molecules controlling follicular growth and oocyte development are in part mediated through extracellular vesicles (EVs), which encompass a highly conserved and advanced paracrine signaling mechanism important in shuttling unique cargo such as microRNAs (miRNAs). In this study, critical miRNAs for follicular development were identified, proposing novel approaches using EV-mediated miRNA to possibly improve the in vitro technologies outcome in a multitude of species.
