Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and 4 replicants were independently labelled. All labellings/hybridizations included two dye swap replicants. Gerbera 9K cDNA microarrays were used for the hybridizations.
Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and alltogether 8 replicates (including 2 biological replicates with 4 technical replicates each) were independently labelled. Both biological replicates included two dye swap replicates. Gerbera 9K cDNA microarrays were used for the hybridizations.
Project description:Three different developmental stages of ray and disc flowers of gerbera hybrida were compared to each other using gerbera 9K cDNA microarray.
Project description:Total RNA extracted from Phytophthora sojae (strain P6497) and infected soybean hypocotyls (cultivar Harosoy) provided template for synthesis of cDNA probes used in the microarray hybridizations. Infected plant hypocotyls were sampled 6 h, 12 h, 24 h, and 48 h after inoculation. Mycelia were grown on synthetic media (H&S) or vegetable juice media (V8). Zoospores were sampled at 0 h, 2 h and 6 h after inducing encystment and germination by agitation. We used microarrays to characterize gene expression patterns in the root rot pathogen Phytophthora sojae and its host Glycine max. Keywords: infection time course, zoospore germination time course, media formulation response
Project description:In this study, we used the illumina high throughput sequencing approach (Sequencing-By-Synthesis, or SBS) to develop the sequence resource of black pepper. To identify micro RNAs functioning in stress response of the black pepper plant, small RNA libraries were prepared from the leaf and root of Phytophthora capsici infected plants, leaves from drought stressed and control plants.
Project description:Transcriptome response of avocado roots subjected to flooding, infection by the oomycete Phytophthora cinnamomi or a combination of both. Analysis was carried out at two time-points. Aim was to identify important genes in response to these stresses.
