Project description:In the microarray experiment a comparison of different flower organs of transformed transgenic gerbera line to wild type gerbera was done. In the transgenic gerbera line the gene gmyb10 that regulates anthocyanin pathway is over-expressed. In this line anthocyanin pigmentation is altered compared to the wild type. In this experiment RNA from dark red callus, two stage of petal development and stamens of the transgenic lines were compared to RNA of wild type in order to identify target genes for gmyb10.
Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and 4 replicants were independently labelled. All labellings/hybridizations included two dye swap replicants. Gerbera 9K cDNA microarrays were used for the hybridizations.
Project description:Gerbera inflorescence sample was compared with a gerbera leaf sample in order to identify flower specific genes. Samples were pooled from different developmental stages and alltogether 8 replicates (including 2 biological replicates with 4 technical replicates each) were independently labelled. Both biological replicates included two dye swap replicates. Gerbera 9K cDNA microarrays were used for the hybridizations.
Project description:Three different developmental stages of ray and disc flowers of gerbera hybrida were compared to each other using gerbera 9K cDNA microarray.
Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None.
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..
Project description:Different gerbera hybrida flower organs including pappus bristles, stamen, early and late petal development and flower scape, were compared to a pooled reference sample to determine which genes are abundant for analyzed flower organs. Samples were pooled and three replicants that were independently labelled were included. All labellings/hybridizations included one dye swap technical repat. Gerbera 9K cDNA microarrays were used for hybridizations.
