Project description:scRNAseq from liver was performed for two Li-TSC1KORagAGTP and two wild-type mice to examine different populations of hepatic cells

2024-01-12 | GSE229830 | GEO

PRC2 proteins EZH1 and EZH2 regulate postnatal hepatic maturation [RNA-Seq]

Project description:The inability to derive fully functional cell types, such as hepatocytes, from stem cells may emanate from the lack of knowledge about mechanisms that underlie postnatal cell maturation. We characterized hepatic maturation during the postnatal day 14 (P14) to 2-month-old (M2) transition and found more than 3000 genes differentially expressed. Nearly half of such maturation genes have H3K27me3 at their promoters or gene bodies at P14 or M2. Genetic ablation of both PRC2 histone methyltransferases in perinatal livers causes hepatocytes to prematurely differentiate, expressing genes at P14 that would normally be induced later, by M2. Using srHC-seq, a new method to map sonication-resistant heterochromatin, we found that the H3K27me3+ prematurely upregulated genes have euchromatic promoters at P14. We also observed derepression of many non-hepatic lineage genes with euchromatic promoter H3K27me3-marking. Thus, Polycomb repression is used to restrain expression of late liver maturation genes and lineage inappropriate genes at euchromatic promoters.

2019-03-01 | GSE118757 | GEO

PRC2 proteins EZH1 and EZH2 regulate postnatal hepatic maturation [srHC-seq]

2019-03-01 | GSE119218 | GEO

PRC2 proteins EZH1 and EZH2 regulate postnatal hepatic maturation [ChIP-Seq]

2019-03-01 | GSE119112 | GEO

PRC2 proteins EZH1 and EZH2 regulate postnatal hepatic maturation [RNA-seq2]

2019-03-01 | GSE125526 | GEO

Li-treatment-induced NDI (Li-NDI) in rats

Project description:5 rats were offered food containing 40mM Li/Kg dry weight for 4 weeks, and 5 control rats obtained standard food. The RNA from the inner medulla of the Li-treated rats was labeled red (channel 1), and from the control rats labeled green (channel 2). The samples from Li-treated rats 1+2 and control rats 1+2 were hybridized to array Li-NDI 1. The samples from Li-treated rats 3+4 and control rats 3+4 were hybridized to array Li-NDI 2. The samples from Li-treated rat 5 and control rat 5 were hybridized to array Li-NDI 3. Keywords: parallel sample

2004-09-01 | GSE1341 | GEO

Gene expression data from livers of Yap+/+ and Yap+/- mice at postnatal day 30

Project description:Liver undergoes both size increase and differentiation during postnatal period, which in mice is approximately first 30 days. The mechanisms of simultaneous postnatal liver cell proliferation and maturation are not clear. In these experiments, role of yes associated protein (Yap), the downstream effector of Hippo Kinase signaling pathway was investigated. Total RNA isolated from livers of Yap+/+ and Yap+/- mice at Postnatal day 30. Pooled livers from 5 mice per genotype were used.

2012-04-04 | E-GEOD-31281 | biostudies-arrayexpress

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