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In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [RNA-Seq 2]

ABSTRACT: In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [RNA-Seq 2]

PROVIDER: PRJNA1010845 | ENA |

REPOSITORIES: ENA

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In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity

Project description:In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity

| PRJNA1010842 | ENA

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [miRNA-Seq]

Project description:The ovarian cortical reserve of follicles is vital for fertility. Some medical treatments are toxic to follicles, leading to premature ovarian insufficiency. Ovarian tissue cryopreservation is an established method to preserve fertility in adults and even applied in prepuberty despite unproven efficacy. Here, we analyze transcriptomes of 120 cortical follicles from children and adults for detailed comparison. We discover heterogeneity with two main types of follicles in both age groups: one with expected oocyte-granulosa profiles and another with predicted role in signaling. Transcriptional changes during growth to the secondary stage are similar overall in children and adults, but variations related to extracellular matrix, theca cells, and miRNA profiles are found. Notably, cyclophosphamide dose correlates with interferon signaling in child follicles. Additionally, morphology alone is insufficient for follicle categorization suggesting a need for additional markers. Marker genes for early follicle activation are determined. These findings will help refine follicular classification and fertility preservation techniques across critical ages.

2024-06-26 | GSE241983 | GEO

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [RNA-Seq 1]

2024-06-26 | GSE241981 | GEO

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [RNA-Seq 2]

2024-06-26 | GSE241982 | GEO

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [miRNA-Seq]

Project description:In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [miRNA-Seq]

| PRJNA1010844 | ENA

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [RNA-Seq 1]

Project description:In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity [RNA-Seq 1]

| PRJNA1010846 | ENA

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity.

| S-EPMC11339373 | biostudies-literature

In-depth analysis of transcriptomes in ovarian cortical follicles from children and adults reveals interfollicular heterogeneity

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-06-26 | GSE241984 | GEO

Establishment of adult epidermal organoid cultures from canine hair follicles and interfollicular epidermis

Project description:Establishment of adult epidermal organoid cultures from canine hair follicles and interfollicular epidermis

| PRJNA413319 | ENA

Establishment of adult epidermal organoid cultures from canine hair follicles and interfollicular epidermis

Project description:The epidermis and associated appendages ensure a number of critical functions necessary for survival and social interactions. Perturbations of epidermal stem cell homeostasis lead to a variety of skin diseases affecting humans and dogs. Therefore, the establishment of an in vitro system to investigate canine epidermal stem cells, representing a model for human diseases, is essential. Here we report the establishment and characterization of organoids derived from microdissected canine hair follicles (HFs) and interfollicular epidermal (IFE). Gene and protein expression analysis revealed high mRNA and protein levels of keratin 5 and 14, IFE differentiation markers and intercellular molecules (e.g., keratin 10 and desmoglein), indicating a strong basal cell signature as well as differentiation towards mature epidermis. Key markers of HF stem cells were lacking. This suggests that, independently of the tissue of origin, both organoid lines develop into the same cell type (basal IFE-like keratinocytes). Signaling pathways members important for regulation of HF growth and cycling (such as Wnt, Hh, BMP and Notch) were present in both HF and IFE organoids at low levels. Withdrawal of growth factors (Noggin, R-spondin and FGFs) resulted in upregulation of markers such as KRT16, IVL, KRT17 and SOX9, showing the potential of the organoids to develop towards more differentiated tissue. However, for induction of HF signatures or hair growth, addition of different growth factors or dermal papilla co-culture might be required. Taken together, our in vitro culture system can provide the basis to address hair growth and explore epidermal function/regeneration, allowing us to further investigate pathomechanisms of cutaneous disorders in dogs and potentially human patients.

2018-07-02 | GSE104622 | GEO

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