Project description:The complete mitochondrial genome of Paratrypauchen microcephalus (Gobiiformes: Oxudercidae) was completely sequenced by high throughput sequencing method. The complete mitochondrial genome was 16,552?bp in length, consisted of 13 protein-coding genes, 22 tRNA genes, two rRNA genes, a putative control region (CR), and an origin of replication on the light-strand (OL). The base composition values for the mitochondrial genome were 29.1%, 27.6%, 15.7%, and 27.6% for A, C, G, and T, respectively. The gene arrangement is identical to those in typical fishes. Phylogenetic tree based on 13 protein-coding genes shows that P. microcephalus has a close phylogenetic relationship with genus Trypauchen and belongs to Oxudercidae.