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ARID3A coordinates the proliferation-differentiation switch of transit-amplifying cells in the intestine

ABSTRACT: ARID3A coordinates the proliferation-differentiation switch of transit-amplifying cells in the intestine

PROVIDER: PRJNA1016446 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26056756.fastq.gz	Fastqsanger.gz
	SRR26056757.fastq.gz	Fastqsanger.gz
	SRR26056758.fastq.gz	Fastqsanger.gz
	SRR26056759.fastq.gz	Fastqsanger.gz
	SRR26056760.fastq.gz	Fastqsanger.gz

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ARID3A coordinates the proliferation-differentiation switch of transit-amplifying cells in the intestine

Project description:Intestinal stem cells (ISCs) at the crypt base divide and give rise to progenitor cells that have the capacity to proliferate and differentiate into various mature epithelial cell types in the transit-amplifying (TA) zone. Here, we identified the transcription factor ARID3A as a novel regulator of intestinal epithelial cell proliferation and differentiation at the TA compartment. We show that ARID3A forms an expression gradient from villus tip to the early progenitors at the crypts mediated by TGF-β and WNT signalling. Intestinal epithelial-specific deletion of Arid3a reduces proliferation of TA cells. Bulk and single cell transcriptomic analysis shows increased enterocyte differentiation and reduced secretory cells in the Arid3a cKO intestine. Interestingly, upper-villus gene signatures of both enterocytes and secretory cells are enriched in the mutant intestine. We find that the enhanced enterocyte differentiation in the Arid3a cKO intestine is caused by increased binding of HNF1 and HNF4. Finally, we show that loss of Arid3a impairs irradiation-induced regenerative process by altering the dynamics of proliferation and apoptosis. Our findings imply that ARID3A may play a gatekeeping role in the TA compartment to maintain the “just-right” proliferation-to-differentiation ratio for tissue homeostasis and plasticity

2024-07-31 | GSE243155 | GEO

Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor [Microarray Expression]

Project description:Regulation of transit amplifying cell formation from self-renewing stem cell is fundamental process for cell replacement in a controlled way. Here we analyse the properties of a population of mesenchymal TACs in the continuously growing mouse incisor to identify key components of the molecular regulation that drives proliferation. Using gene microarray profiling, we show that the polycomb repressive complex 1 acts as a global regulator of the TAC phenotype by its direct action on the expression of key cell cycle regulatory genes and also by regulating Wnt/b-catenin signalling activity. Analysing the properties of mesenchymal transit amplifying cells population and identifing key components of the molecular regulation that drives proliferation.

2019-03-12 | GSE104934 | GEO

Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor [Ring1b ChIP]

Project description:Regulation of transit amplifying cell formation from self-renewing stem cell is fundamental process for cell replacement in a controlled way. Here we analyse the properties of a population of mesenchymal TACs in the continuously growing mouse incisor to identify key components of the molecular regulation that drives proliferation. We show that the polycomb repressive complex 1 acts as a global regulator of the TAC phenotype by its direct action on the expression of key cell cycle regulatory genes and also by regulating Wnt/b-catenin signalling activity. We also identify an essential requirement for TACs in maintaining the mesenchymal stem cells, indicative of a positive feedback mechanism. Analysing the properties of mesenchymal transit amplifying cells population and identifing key components of the molecular regulation that drives proliferation.

2019-03-12 | GSE104892 | GEO

Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor [Histone ChIP]

Project description:Regulation of transit amplifying cell formation from self-renewing stem cell is fundamental process for cell replacement in a controlled way. Here we analyse the properties of a population of mesenchymal TACs in the continuously growing mouse incisor to identify key components of the molecular regulation that drives proliferation. We show that the polycomb repressive complex 1 acts as a global regulator of the TAC phenotype by its direct action on the expression of key cell cycle regulatory genes and also by regulating Wnt/b-catenin signalling activity. We also identify an essential requirement for TACs in maintaining the mesenchymal stem cells, indicative of a positive feedback mechanism. Analysing the properties of mesenchymal transit amplifying cells population and identifing key components of the molecular regulation that drives proliferation.

2019-03-12 | GSE104891 | GEO

Single cell expression profiling of human epidermal stem and transit amplifying cells

Project description:To explore which signaling pathways are of importance in maintenance of human epidermal keratinocytes we have established a protocol which allows single cell expression profiling. The protocol was applied to cultured human epidermal keratinocytes, and single cell cDNA libraries from stem and transit amplifying cells were identified based on the expression of known SC markers, MCSP and Dll1. We used microarrays to obtain expression profiles from single cells and identify common denominators that could be important for maintenance and signalling in epidermal stem cells. Keywords: Timecourse

2006-08-04 | GSE4858 | GEO

CMK LC-MS/MS after CoIP of ARID3A

Project description:In order to determine proteins interacting with ARID3A in the ML-DS cell line CMK, we performed CoIP of endogenous ARID3A followed by LC-MS/MS

2023-03-11 | PXD025027 | Pride

TRANSIT

Project description:TRANSIT

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Bi-directional interaction between mesenchymal stem cells and transit amplifying cells in mesenchymal tissue homeostasis

Project description:Bi-directional interaction between mesenchymal stem cells and transit amplifying cells in mesenchymal tissue homeostasis

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Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor

Project description:Regulation of mesenchymal stem to transit amplifying cell transition in the continuously growing mouse incisor

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RNA-seq of mouse Lgr5 expressing intestinal stem cells (ISCs) and transit amplifying (TA) cells

Project description:RNA-seq of mouse Lgr5 expressing intestinal stem cells (ISCs) and transit amplifying (TA) cells