Project description:This SuperSeries is composed of the following subset Series: GSE31525: Spider mite preliminary feeding experiment with mites reared on bean and two Arabidopsis thaliana accessions GSE31527: Developmental stage-specific gene expression in the two-spotted spider mite (Tetranychus urticae) GSE32005: Developmental stage-specific small RNA composition in the two-spotted spider mite (Tetranychus urticae) GSE32009: Transcriptional responses of the two-spotted spider mite (Tetranychus urticae) after transfer to different plant hosts Refer to individual Series
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of differentially expressed genes (DEG) between the treated samples of mites.The goal of the study is to elucidate the invasion mechanism that results in the continuous expansion of Tetranychus urticae that has occurred in China. Methods:Transcriptome profiling by RNA sequencing is becoming an attractive method as it facilitates rapid generation, identification and quantification of large number of transcripts, even in the species where no prior genome sequence information is available. Results:the expression of 10 P450(cytochrome P450 monooxygenases), 6 GST(glutathione S-transferases), 13 CCE(carboxy/cholinesterases) and other novel candidate genes was higher (>2-fold) in T. urticae than in T. cinnabarinus as detected by using the digital gene expression (DGE) method.
Project description:While performing mitochondrial isolations and recently developed tRNA-seq methods (AlkB treatment and YAMAT-Seq) in plant tissue, we inadvertently sequenced the mitochondrial tRNAs from a common plant pest, the acariform mite Tetranychus urticae, to a high enough coverage to detect all previously annotated T. urticae tRNA regions. The results not only confirm expression, CCA-tailing and post-transcriptional base modification of these highly divergent tRNAs, but also revealed paired sense and antisense expression of multiple T. urticae mitochondrial tRNAs.
Project description:The extreme generalist two-spotted spider mite, Tetranychus urticae, which is documented to feed on more than 1100 plant hosts, is becoming an increasingly important agricultural pest. Historically, as studies of plant-herbivore interactions have focused largely on insects, considerably less research has investigated plant responses to spider mite herbivores, especially in grasses. To identify intraspecific differences in maize response to T. urticae, we collected RNA-seq data from three maize (Zea mays) inbred lines (B73, B75 and B49) as well as two F1 lines arising from crosses between B73 x B75 and B73 x B96. For each maize line, RNA-seq data was collected from uninfested leaves (control) and leaves infested with T. urticae for 24 hours.
