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Multi-omics study identifies unique metabolic responses to high glycemic diet in liver and retina

ABSTRACT: Multi-omics study identifies unique metabolic responses to high glycemic diet in liver and retina

PROVIDER: PRJNA1020144 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26151800_1.fastq.gz	Fastqsanger.gz
	SRR26151800_2.fastq.gz	Fastqsanger.gz
	SRR26151801_1.fastq.gz	Fastqsanger.gz
	SRR26151801_2.fastq.gz	Fastqsanger.gz
	SRR26151802_1.fastq.gz	Fastqsanger.gz

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Multi-omics study identifies unique metabolic responses to high glycemic diet in liver and retina

Project description:High glycemic index (HGI) diet induces hyperglycemia, an etiologic risk factor in major diseases affecting multiple organ systems. We hypothesized that adaptation to dietary glycemic exposure is tissue-specific and depends on the length of intake. We therefore evaluated transcriptomic responses to HGI diet after one or 12-months in the liver and retina of C57BL/6J wild-type mice. In the liver, the genes associated with inflammation and fatty acid metabolism were altered within one-month of HGI diet, whereas 12-month HGI diet-fed group showed dysregulated expression of multiple cytochrome P450 genes and overexpression of key lipogenic factors including Srebf1 and Elovl5. In contrast, retinal transcriptomes exhibited fewer HGI-related changes after one-month, and notable alterations in energy metabolism genes were apparent after 12-months of HGI diet. Fatty acid profiles of liver samples corroborated transcriptomic trends and revealed elevated levels of monounsaturated fatty acid in the HGI group. Conversely, saturated fatty acids and polyunsaturated fatty acids were lower in livers of HGI fed animals, regardless of length of exposure. HGI also resulted in a significant increase in blood low-density lipoprotein (LDL) and overall cholesterol levels. We also uncovered diet-aging interactions affecting expression of mitochondrial oxidative phosphorylation genes in the liver, and inherited disease-associated genes in the retina. Our findings provide new insights into tissue-specific adaptive mechanisms, focusing on the liver and the retina, to dietary hyperglycemia.

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