Project description:This dataset comprises bulk RNA sequencing of 45 samples isolated from adult female L. sclopetarius spiders. The samples include spider abdomen (5 replicates), head (5), major ampullate glands (5), minor ampullate glands (5), aggregate glands (5), tubuliform glands (3), and major ampullate gland cut into three parts, i.e., tail (6), sac (6) and duct (5).
Project description:Female adult spongy moths vary in their flight capacity depending upon their origin and subspecies. Asian spongy moth (Lymantria dispar asiatica) females (such as the RM strain here) can fly whereas European spongy moth (Lymantria dispar dispar) females cannot (such as the CT strain here). We hypothesized that the genetic origins of these differences in flight capacity would be reflected in differences in gene expression during metamorphosis (the pupal stage). To test this, we compared the gene expression at days 1, 3, 5, 8, and 11 of pupal development of female pupae from CT and RM strains.
Project description:Female BALB/c nude mice (n=3/group) were subjected to partial body irradiation under anesthesia using 2 Gy external photon (4 MV, nominal) irradiation. In group A, the collum (i.e. the thyroid) was irradiated; in group B, thorax+abdomen were irradiated, and in group C, collum+thorax+abdomen were irradiated. The control group (n=5) was anesthetized but not irradiated. At 24 h after treatment, the kidneys, liver, lungs, spleen, and thyroid were excised, flash-frozen, and stored at -80°C. Total RNA was extracted from homogenized tissue samples (kidney cortex and kidney medulla were treated separately) and subjected to expression analysis using RNA microarray technology.
Project description:To identify female sex pheromone biosynthesis genes by differential expression between males and females. Female pheromone gland tissue and male abdominal tip tissue was compared by RNA-Seq of cDNA. Transcripts were assembled using Trinity and differential expression analysis done with RSEM.
