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Effect of agarooligosaccharide on gene expression of Ruminococcus gnavus

ABSTRACT: Effect of agarooligosaccharide on gene expression of Ruminococcus gnavus

PROVIDER: PRJNA1020194 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR26151736_1.fastq.gz	Fastqsanger.gz
	SRR26151736_2.fastq.gz	Fastqsanger.gz
	SRR26151737_1.fastq.gz	Fastqsanger.gz
	SRR26151737_2.fastq.gz	Fastqsanger.gz
	SRR26151738_1.fastq.gz	Fastqsanger.gz

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Effect of agarooligosaccharide on gene expression of Ruminococcus gnavus

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Ruminococcus gnavus TSDC16.1-3.1

Project description:Strain Ruminococcus gnavus TSDC16.1-3.1 (species Ruminococcus gnavus) was isolated from the fecal microbiota of a USA female at time point zero . The species name was assigned by genome clustering.

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Project description:This study investigates the growth of Ruminococcus gnavus strain ATCC 29149 under two different conditions over a period of 3 hours. The experimental conditions include: RG (Ruminococcus gnavus in Meat Broth): R. gnavus grown in standard meat broth. RS-SA (Ruminococcus gnavus in Meat Broth with Sialic Acid): R. gnavus grown in meat broth supplemented with sialic acid. Control conditions were also established for comparison: CT (Control in Meat Broth): Standard meat broth without R. gnavus. CT-SA (Control in Meat Broth with Sialic Acid): Meat broth with sialic acid, also without R. gnavus.

2024-06-20 | MSV000095089 | GNPS

Bulk RNA-seq of mouse ileum of Lactobacillus rhamnosus GG or Ruminococcus gnavus monoassociated mice.

Project description:Lacticaseibacillus rhamnosus GG (LGG) is a widely consumed probiotic whose potential beneficial effects in humans have been examined in over 250 clinical trials. However, the mechanisms by which LGG modulates host gut physiology remains unknown. R. gnavus is a pathobiont that is strongly associated with inflammatory bowel diseases. Germ free mice were mono-associated with L. rhamonosus (LGG) or R. gnavus (RG) for 21 days, ileum bulk RNA-seq were performed to compare the transcriptomic profiles of LGG or RG associated mice with the transcriptome of germ-free mouse ileum.

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RNA-seq of Ruminococcus bromii L2-63 and Ruminococcus gnavus ATCC 29149 grown in mono- or co-culture with different carbon sources (glucose, soluble or resistant starch)

Project description:The goal was to assess the impact of carbon source and cross-feeding on transcription profile of both bacteria. R. gnavus was grown in monoculture with glucose (Glc). R. bromii was grown in monoculture with soluble (SS) or resistant (RS) starch. R. bromii and R. gnavus were co-cultured with SS or RS. Total RNA was extracted from a culture sample taken at mid- to late exponential phase of growth. rRNA was depleted and mRNA sequenced. 3 biological replicates were prepared for each condition.

2019-07-31 | E-MTAB-7138 | biostudies-arrayexpress

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