Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell profiling of premature neonate airways reveals a continuum of myeloid differentiation

ABSTRACT: Single-cell profiling of premature neonate airways reveals a continuum of myeloid differentiation

PROVIDER: PRJNA1020976 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-Cell Profiling of Premature Neonate Airways Reveals a Continuum of Myeloid Differentiation

Project description:Single-cell genomic technologies hold great potential to advance our understanding of lung development and disease. A major limitation lies in accessing intact cells from primary lung tissues for profiling human airway health. Sampling methods such as endotracheal aspiration that are compatible with clinical interventions could enable longitudinal studies, the enrollment of large cohorts, and the development of novel diagnostics. To explore single-cell RNA sequencing profiling of the cell types present at birth in the airway lumen of extremely premature neonates (<28 wk gestation), we isolated cells from endotracheal aspirates collected from intubated neonates within the first 1 hour after birth. We generated data on 10 subjects, providing a rich view of airway luminal biology at a critical developmental period. Our results show that cells present in the airways of premature neonates primarily represent a continuum of myeloid differentiation, including fetal monocytes (25% of total), intermediate myeloid populations (48%), and macrophages (2.6%). Applying trajectory analysis to the myeloid populations, we identified two trajectories consistent with the developmental stages of interstitial and alveolar macrophages, as well as a third trajectory presenting an alternative pathway bridging the distinct macrophage precursors. The three trajectories share many dynamic genes (N= 5,451), but also have distinct transcriptional changes (259 alveolar-specific, 666 interstitial-specific, and 285 bridging-specific). Overall, our results define cells isolated within the so-called “golden hour of birth” in extremely premature neonate airways, representing complex lung biology, and can be used in studies of human development and disease.

2023-11-14 | GSE208328 | GEO

Single-cell profiling of premature neonate airways reveals a continuum of myeloid differentiation

Project description:Single-cell profiling of premature neonate airways reveals a continuum of myeloid differentiation

| PRJNA1020977 | ENA

Single-Cell Profiling of Premature Neonate Airways Reveals a Continuum of Myeloid Differentiation

Project description:Single-Cell Profiling of Premature Neonate Airways Reveals a Continuum of Myeloid Differentiation

| PRJNA859193 | ENA

Escherichia coli strain:Neonate | isolate:Neonate

Project description:Escherichia coli strain:Neonate | isolate:Neonate Genome sequencing

| PRJNA756168 | ENA

Neonate and Incubator Study

Project description:Neonate and Incubator

| PRJEB47091 | ENA

Alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) patient neonate lung gene expression analysis

Project description:Analysis of genes dysregulated in the lungs of neonates with ACDMPV neonate lungs compared to control lungs

2014-03-01 | GSE54780 | GEO

Epigenetic modules governing skin epidermal homeostasis [neonate skin epidermis]

Project description:Skin homeostasis is guided by spatiotemporal regulation of gene expression, establishing each stage of keratinocyte differentiation. The current study presents transcriptomic and chromatin profiling for the proliferating (basal) and differentiated (suprabasal) cell populations derived from neonate mice (p0-p2) skin epidermis. This multi-omic approach will enable idenitification of cell specific epidermal cross-talk central to the equlibirum.

2023-01-13 | GSE197018 | GEO

Transcriptome profiling across the cell wall expansion continuum in the Arabidopsis primary stem

Project description:Comparison of full-genome transcriptome profiles of four stages along the cell wall expansion continuum of the elongating inflorescence primary stem of Arabiopdis thaliana; plants ranging in height from 10-15cm.

2012-01-26 | E-MEXP-3525 | biostudies-arrayexpress

land-sea continuum microcosm

Project description:land-sea continuum microcosm Raw sequence reads

| PRJNA803024 | ENA

Somatosensory prediction in the premature neonate brain.

Project description:Sensory prediction (SP) is at the core of early cognitive development. Impaired SP may be a key to understanding the emergence of neurodevelopmental disorders, however there is little data on how and when this skill emerges. We set out to provide evidence of SP in the brain of premature neonates in the fundamental sensory modality: touch. Using Diffuse Correlation Spectroscopy, we measured blood flow changes in the somatosensory cortex of premature neonates presented with a vibrotactile stimulation-omission sequence. When ISI was fixed, participants presented a decrease in blood flow during stimulus omissions, starting when a stimulus should begin: the expectation of a certain stimulus onset induced deactivation of the somatosensory cortex. When ISI was jittered, we observed an increase in blood flow during omissions: the expectation of a likely but not certain stimulus onset induced activation of the somatosensory cortex. Our results reveal SP in the brain as early as four weeks before term, based on the temporal structure of a unimodal somatosensory stimulation, and show that SP produces opposite regulation of activity in the somatosensory cortex depending on how liable is stimulus onset. Future studies will investigate the predictive value of somatosensory prediction on neurodevelopment in this vulnerable population.

| S-EPMC9428805 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data