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Role of Mac1 in gene regulation under copper limiting conditions in Fusarium oxysporum [ChIP-seq]

ABSTRACT: Role of Mac1 in gene regulation under copper limiting conditions in Fusarium oxysporum [ChIP-seq]

PROVIDER: PRJNA1021232 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26184560_1.fastq.gz	Fastqsanger.gz
	SRR26184560_2.fastq.gz	Fastqsanger.gz
	SRR26184561_1.fastq.gz	Fastqsanger.gz
	SRR26184561_2.fastq.gz	Fastqsanger.gz
	SRR26184562_1.fastq.gz	Fastqsanger.gz

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Role of Mac1 in gene regulation under copper limiting conditions in Fusarium oxysporum [ChIP-seq]

Project description:The transcription factor Mac1 is a key regulator of copper homeostasis and controls the transcriptional response to copper-limiting conditions in fungi. Expression analyses performed in the soil-borne plant pathogen Fusarium oxysporum revealed that almost all copper starvation-induced genes are downregulated in the absence of the regulator Mac1. The aim of this ChIP-seq analysis is to elucidate which of these genes are direct targets of Mac1.

2024-11-07 | GSE244102 | GEO

Effect of Mac1 deletion on copper homoestasis and virulence in Fusarium oxysporum f. sp. lycopersici [RNA-seq]

Project description:The transcription factor Mac1 is a key regulator of copper homeostasis, controlling the transcriptional response to copper-limiting conditions in fungi. It has been described as a virulence factor in human pathogens such as Candida albicans and Aspergillus fumigatus, but surprisingly its role in plant pathogenesis is poorly understood. Targeted deletion of Mac1 in the soilborne pathogen Fusarium oxisporum results in an inability to grow under copper-limited conditions and kills tomato plants. The aim of this RNA-seq analysis is to elucidate the role of Mac1 in gene regulation under copper starvation and during infection of tomato plants by F. oxysporum.

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Comparative O-glycoproteomics of Fusarium oxysporum

Project description:Fusarium oxysporum causes Fusarium wilt syndrome in more than 120 different plant hosts, including globally important crops such as tomato, cotton, banana, melon, etc. F. oxysporum shows high host specificity in over 150 formae speciales and have been ranked in the top 10 plant fungal pathogens. Although three PMTs encoded by the pmt1, pmt2, and pmt4 are annotated in the genome of F. oxysporum, their functions have not been reported. As O-mannosylation is not found in plants, a comprehensive understanding of PMTs in F. oxysporum becomes attractive for the development of new strategy against Fusarium wilt. In order to understand the molecular mechanism of the differential functions of three PMTs, a comparative O-glycoproteome analysis of the pmt mutants were carried out.

2019-10-28 | PXD012530 | Pride

Expression data from Capsicum annuum inoculated with Fusarium oxysporum Fo47

Project description:The colonization of Capsicum annuum roots by Fusarium oxysporum Fo47 induces resistance responses on the plant. Fo47 is a non-pathogenic strain of Fusarium oxysporum. Fo47 colonizes only the most outer layers of the root surface but it does not colonize inner tissues. Pre-treatment of roots with Fo47 reduces the symptom development produced by later pathogen inoculation. The expression of genes in distal tissues was determined by microarray analysis of stems of Fo47-treated plants. Capsicum annuum samples were analyzed using Affymetrix chips of the close-related species Solanum lycopersicum.

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Genome wide expression responses of Fusarium oxysporum wt and M-NM-^ThapX mutant to iron depleted and iron replete conditions.

Project description:Soilborne fungal pathogens cause devastating yield losses, are highly persistent and difficult to control. To culminate infection, these organisms must cope with limited availability of iron. Here we show that the bZIP protein HapX functions as a key regulator of iron homeostasis and virulence in the vascular wilt fungus Fusarium oxysporum. Deletion of hapX does not affect iron uptake, but causes derepression of genes involved in iron-consuming pathways, leading to impaired growth under iron-depleted conditions. F. oxysporum strains lacking HapX are reduced in their capacity to invade and kill tomato plants and immunodepressed mice. The virulence defect of M-NM-^ThapX on tomato plants is exacerbated by coinoculation of roots with a biocontrol strain of Pseudomonas putida, but not with a siderophore-deficient mutant, indicating that HapX contributes to iron competition of F. oxysporum in the tomato rhizosphere. These results establish a conserved role for HapX-mediated iron homeostasis in fungal infection of plants and mammals. Iron dependent gene expression in Fusarium oxysporum wt and M-NM-^ThapX mutant was measured 1 hour after shifting the mycelia to minimal medium with or without 50 M-NM-<M Fe2(SO4)3. Three independent experiments were performed.

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Expression profiling of Fusarium oxysporum infected wild-type Arabidopsis and a 35S Fusarium SIX4 overexpression line.

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Genome Sequencing and Comparative Transcriptomics of Race1 and Race4 of Banana pathogen: Fusarium oxysporum f.sp. Cubense

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Effect of Mac1 deletion on copper homoestasis and virulence in Fusarium oxysporum f. sp. lycopersici [RNA-seq]

Project description:Effect of Mac1 deletion on copper homoestasis and virulence in Fusarium oxysporum f. sp. lycopersici [RNA-seq]

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Cotton (Gossypium barbadense) root transcriptional response to Fusarium oxysporum f.sp. vasinfectum (FOV) race 4 (FOV4) infection

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Expression profiling of Fusarium oxysporum infected wild-type Arabidopsis and a 35S Fusarium SIX4 overexpression line.

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