Project description:Microarray expression profiling of cervical exfoliated cells (10k genes) We used whole genome microarrays (25,353 unique genes) to compare the transcription profiles from seven samples of normal exfoliated cells and one cervical tissue.
Project description:Mesenchymal stromal stem cells from human bone marrow (BMSCs), adipose tissuse (ADSCs) were purchased from ATCC. Human exfoliated deciduous dental pulps were cultured in vitro and purified to get stem cells from human exfoliated deciduous teeth (SHED). Markers of MSCs were checked before scRNA seq.
Project description:Profiling of chromosomal gains and losses in a genomic complement of stem cells from human exfoliated deciduous teeth comparing control undifferentieated SHED with hepatocyte differentiated SHED.
Project description:To investigate the different gens expression and RNA methylation degree in the cervical immortalized cervical cells and cervical cancer cells，
Project description:To explore the circRNA expression profiles during the development and progression of cervical cancer, we performed RNA sequencing analysis with ribosomal RNA-depleted in HPV negative normal cervical epithelium, HPV16 positive normal cervical epithelium, HPV16 positive high-grade squamous intraepithelial lesion (HSIL), and HPV16 positive cervical squamous cell carcinoma tissues,6 cases in each group.Totally 66868 circRNAs were identified (Back-spliced junctions reads≥1)
2020-12-21 | GSE147009 | GEO
Project description:RNA sequence in osteo/odontogenesis of stem cells from human exfoliated deciduous teeth
Project description:This is a prospective observational case-control study conducted in septic VLBW dizygotic twins and their non-septic twin controls. Fecal samples were used for genome-wide expression analysis of exfoliated intestinal cells.
Project description:bFGF regulated stemness maintenance and cell differentiation in stem cells isolated from human exfoliated deciduous teeth (SHEDs). In addition, WNT3A is also shown to regulate osteogenic differentiation in mesenchymal stem cells. Here, transcriptome analysis of bFGF and WNT3A treated SHEDs was evaluated using a high throughput RNA sequencing technique. Results demonstrated that bFGF regulated genes related to cell cycle control and cell proliferation in SHEDs.
