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ABSTRACT: RNA sequence in osteo/odontogenesis of stem cells from human exfoliated deciduous teeth

PROVIDER: PRJNA647828 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12285690_1.fastq.gz	Fastqsanger.gz
	SRR12285690_2.fastq.gz	Fastqsanger.gz
	SRR12285691_1.fastq.gz	Fastqsanger.gz
	SRR12285691_2.fastq.gz	Fastqsanger.gz
	SRR12285692_1.fastq.gz	Fastqsanger.gz

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Gene Profiles of Mesenchymal Stromal Cells from Different Tissue

Project description:Mesenchymal stromal stem cells from human bone marrow (BMSCs), adipose tissuse (ADSCs) were purchased from ATCC. Human exfoliated deciduous dental pulps were cultured in vitro and purified to get stem cells from human exfoliated deciduous teeth (SHED). Markers of MSCs were checked before scRNA seq.

2023-09-12 | GSE157863 | GEO

SHED: control vs Hepatocyte-like cells

Project description:Profiling of chromosomal gains and losses in a genomic complement of stem cells from human exfoliated deciduous teeth comparing control undifferentieated SHED with hepatocyte differentiated SHED.

2018-02-01 | GSE102974 | GEO

Transcriptome analysis of bFGF and WNT3A treated stem cells isolated from human exfoliated deciduous teeth

Project description:bFGF regulated stemness maintenance and cell differentiation in stem cells isolated from human exfoliated deciduous teeth (SHEDs). In addition, WNT3A is also shown to regulate osteogenic differentiation in mesenchymal stem cells. Here, transcriptome analysis of bFGF and WNT3A treated SHEDs was evaluated using a high throughput RNA sequencing technique. Results demonstrated that bFGF regulated genes related to cell cycle control and cell proliferation in SHEDs.

2020-07-07 | GSE131758 | GEO

Inorganic Pyrophosphate influence Stem Cells from Human Exfoliated Deciduous Teeth

Project description:Inorganic Pyrophosphate influence Stem Cells from Human Exfoliated Deciduous Teeth

| PRJNA1185148 | ENA

Proteomics of exosomes derived from rat trigeminal ganglion neurons

Project description:Exosomes derived from rat trigeminal ganglion neurons (TGN) were harvested as control group. Exosomes derived from TGN after internalizing exosomes derived from stem cells from human exfoliated deciduous teeth (S-Exo) were harvested as treatment group.

2024-01-22 | PXD048746 |

Identification of gene related to stem cells from human exfoliated deciduous teeth (SHED) into epithelial-like differentiation using co-culture in epithelial microenvironment

Project description:We employed microarray profiling to determine the gene(s) that control human exfoliated deciduous teeth (SHED) differentiation into epithelial-like cells. We introduced epithelial microenvironment using keratinocyte serum free medium (KSFM) and co-culture with human epithelial keratinocyte (HEK001) to promote epithelial-like differentiation.

2024-02-01 | GSE165451 | GEO

Inorganic Pyrophosphate influence Stem Cells from Human Exfoliated Deciduous Teeth

Project description:To explore the cellular response of stem cell derived from human exfoliated deciduous teeth (SHED) in response to inorganic pyrophosphate (PPi). SHED was extracted from human primary exfoliated teeth. The cellular responses including cell proliferation, apoptosis, migration, transcriptomic expression was investigated afterward PPi treatment at the distinct concentration. Additionally, Alizarin red, oil red O, and alcian blue staining were performed to evaluate the multipotency. Inorganic pyrophosphate (PPi) had no although effect on cell cycle, yet enhanced migratory cell, compared to control. Additionally, PPi abolished the gene expression of osteogenic genes and also calcium deposition. Interestingly, the inhibition of mineralization by PPi was not reversed though Pi3K and MAPK family, including ERK, P38, and JNK signalling pathway. On the contrary, PPi induced the ALPL and COL1A1 gene expression and reduced RANKL mRNA and protein in the condition medium, resulting in a decrease of osteoclast formation. The transcriptomic profiles illustrated PPi modulated interferon ⍺/ , TGF-β1, NOTCH signalling pathway, and metabolism of lipid. In summary, our study revealed that PPi enhanced migratory ability, however inhibited osteo/odontogenic, adipogenic and chondrogenic differentiation. This study implicated that PPi can modulate cellular responses of SHED.

2024-12-05 | GSE281644 | GEO

stem cells from human exfoliated deciduous teeth cultured with human platelet lysate

Project description:Stem cells from human exfoliated deciduous teeth (SHED) can be used as a potential clinical material. But the use of xenogeneic ingredients, especially fetal bovine serum, will increase the risk of zoonotic disease transmission during the use of animal-derived products. Human-platelet-lysate (hPL) was used in human cell expansion with reliability in clinical applications. This study aimed to explore the feasibility and effect of HPL in the cultivation of SHED. We use proteomics to interpret the impact of HPL on the protein profile of SHED. Propagation, differentiation, qPCR and immunofluorescence were used to detect the effect of HPL on SHED.

2022-05-19 | PXD026687 | Pride

Transcriptome analysis of bFGF and WNT3A treated stem cells isolated from human exfoliated deciduous teeth

Project description:Transcriptome analysis of bFGF and WNT3A treated stem cells isolated from human exfoliated deciduous teeth

| PRJNA544695 | ENA

Molecular differences between mesenchymal populations from deciduous and permanent human teeth

Project description:Deciduous and permanent human teeth represent a model system to study ageing of mesenchymal populations. Aging is tightly connected to self-renewal and proliferation and thus, mapping potential molecular differences in these characteristics between populations constitutes an important task. Specifically designed microarray panels were used. We have detected a number of molecules that were differentially expressed in dental pulp mesenchyme from deciduous and permanent teeth extracted from young children and adults, respectively. Among the differentially regulated genes HMGA2, a stem cell-associated marker, stood out as a remarkable example with a robust expression in deciduous pulp cells. In addition to this, we discovered that several proliferation-related genes, including CDC2A and CDK4, were up-regulated in deciduous pulp cells, while matrix genes COL1A1, fibronectin and several signaling molecules, such as VEGF, FGFr-1 and IGFr-1 were up-regulated in the pulp cells from permanent teeth. Taken together, our data suggest that deciduous pulp cells are more robust in self- renewal and proliferation, whereas adult dental pulp cells are more capable of signaling and matrix synthesis.

2014-09-01 | GSE58668 | GEO

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