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Effect of Hyperbaric Oxygen and Inflammation on Human Gingival Mesenchymal Stem/Progenitor Cells

ABSTRACT: Effect of Hyperbaric Oxygen and Inflammation on Human Gingival Mesenchymal Stem/Progenitor Cells

PROVIDER: PRJNA1022801 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26242963.fastq.gz	Fastqsanger.gz
	SRR26242964.fastq.gz	Fastqsanger.gz
	SRR26242965.fastq.gz	Fastqsanger.gz
	SRR26242966.fastq.gz	Fastqsanger.gz
	SRR26242967.fastq.gz	Fastqsanger.gz

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Effect of Hyperbaric Oxygen and Inflammation on Human Gingival Mesenchymal Stem/Progenitor Cells

Project description:The present study explores for the first time the effect of hyperbaric oxygen (HBO) on gingival mesenchymal stem cells’ (G-MSCs) gene expression profile, intracellular pathways activation, pluripotency and differentiation potential under an experimental inflammatory setup. G-MSCs were isolated from five healthy individuals (n=5) and characterized. Single (24h) or double (72h) HBO stimulation (100%O2, 3bar, 90min) was performed under experimental in-flammatory [IL-1β(1ng/ml)/TNF-α(10ng/ml)/IFN-γ(100ng/ml)] and non-inflammatory mi-cro-environment. Next Generation Sequencing and KEGG pathway enrichment analysis, G-MSCs’ pluripotency gene expression, Wnt-/β-catenin pathway activation, proliferation, colo-ny-formation and differentiation were investigated. G-MSCs demonstrated all mesenchymal stem/progenitor cells’ characteristics. The benefi-cial effect of a single HBO stimulation was evident, with anti-inflammatory effects and induction of differentiation (TLL1, ID3, BHLHE40), proliferation/cell survival (BMF, ID3, TXNIP, PDK4, ABL2), migration (ABL2) and osteogenic differentiation (p<0.05). A second HBO stimulation at 72h had a detrimental effect, significantly increasing the inflammation-induced cellular stress and ROS accumulation through HMOX1, BHLHE40 and ARL4C amplification and pathway en-richment (p<0.05). Results outline a positive short-term single HBO anti-inflammatory, regenerative and dif-ferentiation stimulatory effect on G-MSCs. A second (72h) stimulation is yet detrimental on the same properties. The current results could open new perspectives in the clinical application of short-termed HBO induction in G-MSCs-mediated periodontal reparative/regenerative mecha-nisms.

2023-10-05 | GSE244404 | GEO

Hyperbaric Oxygen Therapy in Treating Long-Term Gastrointestinal Adverse Effects Caused by Radiation Therapy in Patients With Pelvic Cancer

Project description:RATIONALE: Radiation therapy can cause long-term adverse effects. Hyperbaric oxygen therapy may be effective in lessening gastrointestinal symptoms caused by radiation therapy given for pelvic cancer. It is not yet known whether high-pressure oxygen is effective in treating adverse effects caused by radiation therapy. PURPOSE: This randomized phase III trial is studying hyperbaric oxygen therapy to see how well it works in treating long-term gastrointestinal adverse effects caused by radiation therapy in patients with pelvic cancer.

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Hyperbaric oxygen therapy after mid-cervical spinal contusion injury

Project description:Hyperbaric oxygen therapy after mid-cervical spinal contusion injury

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Proteome analysis of pig heart tissue to study the molecular consequences of hyperbaric oxygen treatment

2024-06-22 | PXD047678 | Pride

Gene expression analysis of bleomycin-challenged mouse lung tissues with/without repetitive hyperbaric oxygen treatment

Project description:Idiopathic pulmonary fibrosis (IPF) is a dreadful, chronic, and irreversibly progressive disease leading to death with few effective treatments. Our previous study suggested that repetitive hyperbaric oxygen (HBO) treatment alleviates bleomycin-induced pulmonary fibrosis in mice, but the underlying mechanism is not clear. In this study, we compared the RNA-seq data from bleomycin challenged mouse lung tissues with or without repetitive HBO treatment, to figure out the affected biological processes and pathways. Combined with integrative analysis of public data from GEO, our study support HBO treatment as a viable strategy against pulmonary fibrosis.

2022-09-29 | GSE200109 | GEO

Complexome profiling of mitochondrial protein complexes from pig heart to study the molecular consequences of hyperbaric oxygen treatment

Project description:The overall goal of the project was to confirm the presence of different energetic states of mitochondrial subpopulations depending on their subcellular localization within cardiomyocytes, i.e., subsarcolemmal (SSM) and interfibrillar mitochondria (IFM). At the same time, we aimed to elucidate possible respiratory plasticity of mitochondrial states in response to oxygen availability because of treatment with hyperbaric oxygen. The results could form the basis for a more efficient therapy of contractile dysfunction of the heart but also of other diseases if they are due to mitochondrial respiratory dysfunction caused by a lack of tissue oxygenation.

2024-06-22 | PXD035554 | Pride

Hyperbaric oxygen therapy after mid-cervical spinal contusion injury

Project description:Hyperbaric oxygen (HBO) therapy is frequently used to treat peripheral wounds or decompression sickness. Evidence suggests that HBO therapy can provide neuroprotection and has an anti-inflammatory impact after neurologic injury including spinal cord injury (SCI). Our primary purpose was to conduct an unbiased, genome-wide screening of mRNA expression changes in the injured spinal cord after HBO therapy. A mRNA gene array was used to evaluate samples taken from the contused region of the spinal cord following a lateralized mid-cervical contusion injury in adult female rats. HBO therapy consisted of daily, 1-hour sessions (3.0 ATA, 100% O2) initiated on the day of SCI. Gene set enrichment analyses indicated that HBO upregulated genes in pathways associated with electron transport, mitochondrial function, and oxidative phosphorylation, and downregulation genes in pathways associated with inflammation (including cytokines and NF-B) and apoptotic signaling. In a separate cohort, spinal cord histology was performed to verify whether the HBO treatment impacted neuronal cell counts or inflammatory markers. Compared to untreated rats, there was increased NeuN positive cells in the spinal cord of HBO treated rats (p=0.007). Further, staining for anti-ionized calcium binding adaptor protein (Iba-1, a microglial marker) was reduced after HBO (p=0.028). We conclude that HBO therapy, initiated shortly after SCI and continued for 10-days, can alter the molecular signature of the lesioned spinal cord in a manner consistent with an anti-inflammatory and neuroprotective impact.

2022-05-18 | GSE185600 | GEO

Mouse lung gene expression analysis after repetitive hyperbaric oxygen exposure based on RNA-seq

Project description:Hyperbaric oxygen (HBO) is widely applied to treat a number of hypoxia-related diseases. Previous studies focused on the immediate effect of HBO-exposure induced oxidative stress on lungs, but knowledge regarding the chronic effects from repetitive HBO exposure is limited, especially at the gene expression level. We found that repetitive HBO exposure did not alter the morphology of murine lungs. However, by deconvolution of RNA-seq from those mice lungs using CIBERSORTx and the expression profile matrices of 8 mesenchymal cell subtypes obtained from bleomycin-treated mouse lungs, we identify several mesenchymal cell subtype changes. These include increases in Col13a1 matrix fibroblasts, mesenchymal progenitors and mesothelial cell populations and decreases in lipofibroblasts, endothelial and Pdgfrb high cell populations. Our data suggest that repetitive HBO exposure may affect biological processes in the lungs such as response to wounding, extracellular matrix, vasculature development and immune response.

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Oxygen effect on murine progenitor oligodrendrocytes

Project description:Hyperoxia is known to cause cerebral white matter injury in preterm infants with male sex being identified as an independent risk factor for poor neurodevelopmental outcome. We investigated the underlying mechanisms behind such a sex dependent difference by a comaparative protein profiling of male and female murine progenitor oligodendrocytes treated with 3 % or 80% oxygen. We demonstrate that following hyperoxia, the male derived oligodendrocyte progenitor cells (OPCs) are severely affected with respect to their energy metabolism, stress response and especially, maturation as compared to their female counterparts.

2021-09-08 | PXD011348 | Pride

Mouse lung gene expression analysis after repetitive hyperbaric oxygen exposure based on RNA-seq

Project description:Mouse lung gene expression analysis after repetitive hyperbaric oxygen exposure based on RNA-seq

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