Project description:Is is a fundamental evolutionary question which coordinated molecular changes underly adaptation generally and thermal adaptation specifically. Here we profiled the proteome of the Planarian glacial relict species Crenobia alpina. We sampled individuals from an alpine spring, acclimated groups of individuals at 8, 11, 14 and 17 °C for one week and determined their proteome. These results give insight into the molecular mechanisms underlying thermal adaptation and acclimation to cold and warm temperatures.
Project description:Full mitogenomes in the critically endangered kākāpō reveal major post-glacial and anthropogenic effects on neutral genetic diversity
| PRJEB25924 | ENA
Project description:Genomic monitoring of the critically endangered kakapo using targeted nanopore sequencing of environmental DNA
Project description:The adult vertebrate red spotted newt is a champion of regeneration, demonstrating an amazing ability to regenerate damaged organs and tissues back to an uninjured state without the formation of scar or reduction in function. By developing a novel cardiac resection strategy, our group recently demonstrated that newt hearts could morphologically and functionally regenerate, without scarring, within a period of 2-3 months following injury. MicroRNAs (miRs) have been widely publicized as essential post-transcriptional gene regulators in a variety of biological processes, including regeneration. We have conducted a microarray screen for vertebrate miRs, with several candidate miRs showing significant differential expression at important time-points following injury to the newt heart. The newt microRNA expression between uninjured hearts and regenerating hearts, 7 and 21 days post-injury (dpi), was compared by microarray analysis. Three paired samples were analyzed: Uninjured, 7dpi and 21dpi newt hearts. Three arrays were hybridized comparing two-paired samples each time.
Project description:The adult vertebrate red spotted newt is a champion of regeneration, demonstrating an amazing ability to regenerate damaged organs and tissues back to an uninjured state without the formation of scar or reduction in function. By developing a novel cardiac resection strategy, our group recently demonstrated that newt hearts could morphologically and functionally regenerate, without scarring, within a period of 2-3 months following injury. MicroRNAs (miRs) have been widely publicized as essential post-transcriptional gene regulators in a variety of biological processes, including regeneration. We have conducted a microarray screen for vertebrate miRs, with several candidate miRs showing significant differential expression at important time-points following injury to the newt heart. The newt microRNA expression between uninjured hearts and regenerating hearts, 7 and 21 days post-injury (dpi), was compared by microarray analysis.