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Hypoxia inducible microRNA-155 negatively regulates epithelial barrier in Eosinophilic Esophagitis by suppressing Tight Junction Claudin-7

ABSTRACT: Hypoxia inducible microRNA-155 negatively regulates epithelial barrier in Eosinophilic Esophagitis by suppressing Tight Junction Claudin-7

PROVIDER: PRJNA1023925 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26281933_1.fastq.gz	Fastqsanger.gz
	SRR26281933_2.fastq.gz	Fastqsanger.gz
	SRR26281934_1.fastq.gz	Fastqsanger.gz
	SRR26281934_2.fastq.gz	Fastqsanger.gz
	SRR26281935_1.fastq.gz	Fastqsanger.gz

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Hypoxia inducible microRNA-155 negatively regulates epithelial barrier in Eosinophilic Esophagitis by suppressing Tight Junction Claudin-7

Project description:MicroRNA (miRNA)-mediated mRNA regulation directs many homeostatic and pathological processes, but how miRNAs coordinate aberrant esophageal inflammation during eosinophilic esophagitis (EoE) is poorly understood. Here, we report a deregulatory axis where microRNA-155 (miR-155) regulates epithelial barrier dysfunction by selectively constraining tight junction CLDN7 (claudin-7). MiR-155 is elevated in the esophageal epithelium of biopsies from patients with active EoE and in cell culture models. miR-155 localisation using in situ hybridisation (ISH) in patient biopsies, and intra-epithelial compartmentalisation of miR-155 shows expression predominantly within the basal epithelia. Epithelial miR-155 activity was evident through diminished target gene expression in 3D organotypic cultures, particularly in relatively undifferentiated basal cell states. Mechanistically, generation of a novel cell line with enhanced epithelial miR-155 stable overexpression induced a functionally deficient epithelial barrier in 3D air-liquid interface epithelial cultures measured by transepithelial electrical resistance (TEER). Histological assessment of 3D esophageal organoid cultures overexpressing miR-155 showed notable dilated intra-epithelial spaces. Unbiased RNA-sequencing analysis and immunofluorescence determined a defect in epithelial barrier tight junctions and revealed a selective reduction in the expression of critical esophageal tight junction molecule, claudin-7. Together, our data reveal a previously unappreciated role for miR-155 in mediating epithelial barrier dysfunction in esophageal inflammation.

2023-12-12 | GSE244629 | GEO

TaqMan microRNA array of human serum from children with eosinophilic esophagitis

Project description:Serum from children with active and inactive treated eosinophilic esophagitis was analyzed for detection of microRNA Individual serum samples from children with eosinophilic esophagitis were analyzed for detection of microRNA. (n=5 for active EoE and n=5 for inactive EoE)

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Project description:Adult Eosinophilic Esophagitis Registry Atlas

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Project description:Adult Eosinophilic Esophagitis Registry Atlas

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Nanostring nCounter human miRNA assay (v1) of eosinophilic esophagitis

Project description:Nanostring nCounter Human miRNA assay (v1) of esophageal mucosal biopsies from children with eosinophilic esophagitis and controls

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TaqMan microRNA array of human serum from children with eosinophilic esophagitis

Project description:Serum from children with active and inactive treated eosinophilic esophagitis was analyzed for detection of microRNA

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Eosinophilic esophagitis-associated epithelial remodeling may limit esophageal carcinogenesis

Project description:Eosinophilic esophagitis and esophageal squamous cell carcinoma both feature epithelial remodeling in the form of hyperplasia. We used scRNA-Seq to examine the cellular and molecular alterations between these distinct pathologies.

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