Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NHE3-knockout small intestine. Keywords: gene expression comparison
Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NHE3/CFTR double knockout small intestine. Keywords: gene expression comparison
Project description:The objective of this study was to identify changes in gene expression levels between wild-type and NKCC1-knockout small intestine. 6 wild-type and 6 NKCC1-knockout small intestine RNA samples were compared. Total RNA was collected from both male and female 8-week old wild-type and NKCC1-null mice on an inbred FVB\N background. All comparisons were done between wild-type and NHE4-null samples from age- and gender-matched mice.
Project description:Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global programme of colonic gene expression in the absence of functional NHE3. Experiment Overall Design: Whole colon was dissected from 6-8 week old NHE3-deficient mice and their wild-type littermates and total RNA isolated for microarray analysis using Affymetrix murine MOE430 2.0 arrays
