Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

WNK463 effect on JJN3 cells

ABSTRACT: WNK463 effect on JJN3 cells

PROVIDER: PRJNA1024364 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR26282700_1.fastq.gz	Fastqsanger.gz
	SRR26282700_2.fastq.gz	Fastqsanger.gz
	SRR26282701_1.fastq.gz	Fastqsanger.gz
	SRR26282701_2.fastq.gz	Fastqsanger.gz
	SRR26282702_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 44

Similar Datasets

Transcriptomics changes upon WNK463 treatment in JJN3 cells

Project description:We performed RNA-seq in JJN3 human multiple myeloma cells treated with WNK463 to profile global transcriptomic changes

2024-04-16 | GSE244667 | GEO

Transcriptomics changes upon WNK1 knockdown in JJN3 cells

Project description:We performed RNA-seq in JJN3 human multiple myeloma cells with or without WNK1 knockdown to profile global transcriptomic changes

2024-04-16 | GSE244668 | GEO

Transcriptomics changes upon WNK463 treatment in JJN3 cells

Project description:Transcriptomics changes upon WNK463 treatment in JJN3 cells

| PRJNA1024365 | ENA

Transcriptomics changes upon WNK1 knockdown in JJN3 cells

Project description:Transcriptomics changes upon WNK1 knockdown in JJN3 cells

| PRJNA1024367 | ENA

Affymetrix U133Plus2.0 data sets of Human Myeloma Cellline JJN3 with non-specific, scrambled shRNA or CKS1B shRNA

Project description:This series represents Affymetrix U133Plus2.0 data sets of JJN3 transduced with levivirus expressing non-specific scrambled shRNA or CKS1B shRNA Keywords: JJN3 CKS1B shRNA

2006-03-31 | GSE3369 | GEO

RNA-seq analysis after THZ1 treatment in JJN3 and H929

Project description:RNA-seq analysis was performed in JJN3 and H929 to analyze gene expression changes after THZ1 treatment.

2021-02-16 | GSE145889 | GEO

shRNA profiling of human myeloma cell line jjn3 with non-specific, scrambled shrna or cks1b shrna

Project description:This series represents Affymetrix U133Plus2.0 data sets of JJN3 transduced with levivirus expressing non-specific scrambled shRNA or CKS1B shRNA Experiment Overall Design: This series represents Affymetrix U133Plus2.0 data sets of JJN3 transduced with levivirus expressing non-specific scrambled shRNA or CKS1B shRNA

2008-06-12 | E-GEOD-3369 | biostudies-arrayexpress

WNK463 effect on JJN3 cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-04-16 | GSE244670 | GEO

RNA-seq analysis after THZ1 treatment in JJN3 and H929

Project description:RNA-seq analysis after THZ1 treatment in JJN3 and H929

| PRJNA608683 | ENA

Global transcriptional changes in the JJN3 myeloma cell line that occur as a result of treatment with 2 pyrrolobenzodiazepine (PBD) monomers

Project description:Background: This study characterises a group of Pyrrolo[2,1-c][1,4]benzodiazepine (PBD) monomeric hybrids to investigate the global transcriptional changes that occur as a result of treatment in these cells. It was also of interest to determine whether compounds show a selective inhibitory effect on the NF-kB consenus sequence and hence NF-kB signalling. Methods: NF-kB is often overexpressed and over-active in multiple myeloma. Therefore, JJN3 myeloma cells were selected for this investigation. These cells were treated with 2 PBD monomers - DC-1-170 and DC-1-192 - at a concentration of 30nM, in triplicate alongside untreated controls. They were lysed in Trizol reagent (Thermo Fisher) and RNA was extracted using an RNeasy mini-prep kit (Qiagen). Isolated RNA was then taken forward for RNA-seq. Downstream analysis was performed using GenView2 software (in-house analysis tool developed by Peter Giles) and Ingenuity Pathway Analysis (Qiagen). Results: RNA-seq analysis revealed an overall inhibitory effect on gene transcription as a result of treatment with both PBD monomers, with a small proportion of genes experiencing increased transcription. Approximately 80% of genes that were altered were common to both PBD compounds tested. Both PBD compounds also showed significant inhibition of NF-kB signalling. Conclusions: RNA-sequencing confirmed gene set enrichment for NF-κB pathway genes although as expected, other canonical pathways were also affected. This could be linked with the toxicity that these cells experience as a result of treatment with PBD monomer hybrids.

2019-01-22 | GSE125395 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data