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Transcriptomic analysis of Drosophila leg disc associated myoblasts

ABSTRACT: Transcriptomic analysis of Drosophila leg disc associated myoblasts

PROVIDER: PRJNA1027342 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26372765.fastq.gz	Fastqsanger.gz
	SRR26372766.fastq.gz	Fastqsanger.gz
	SRR26372767.fastq.gz	Fastqsanger.gz
	SRR26372768.fastq.gz	Fastqsanger.gz
	SRR26372769.fastq.gz	Fastqsanger.gz

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Transcriptomic analysis of Drosophila leg disc associated myoblasts

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CTCF binding profiles in Drosophila melanogaster 3rd instar larva T1 and T3 leg discs

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CTCF binding profiles in Drosophila melanogaster 3rd instar larva T1 and T3 leg discs

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Project description:We report on changes in gene expression resulting from the ecdysone-directed broad-based transcriptional regulatory hierarchy in Drosophila melanogaster leg imaginal discs by comparing expression in white1118 third-instar larvae (-18 hrs relative to pupariation) and white prepupae (0 hrs) to examine the transcriptional effects of the late larval ecdysone pulse, as well comparing expression in white1118 and broad5 white prepupal leg imaginal discs to examine the transcriptional effects of the early ecdysone responder, broad. Using this approach, we were able to identify critical leg disc elongation genes that are differentially regulated in broad mutants

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Transcriptomic analysis of tendon progenitors of Drosophila leg

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JAK/STAT coordinates cell proliferation during disc regeneration with Dilp8-mediated developmental delay in Drosophila melanogaster

Project description:Regeneration of fragmented Drosophila imaginal discs occurs in an epimorphic manner, involving local cell proliferation at the wound site. Following disc fragmentation, cells at the wound site activate a restoration program through wound healing, regenerative cell proliferation and repatterning of the tissue. However, the interplay of signaling cascades, driving these early reprogramming steps, is not well understood. Here we profiled the transcriptome of regenerating cells in the early phase within twenty-four hours after wounding. We found that JAK/STAT signaling becomes activated at the wound site and promotes regenerative cell proliferation in cooperation with Wingless (Wg) signaling. In addition, we demonstrated that the expression of Drosophila insulin-like peptide 8 (dilp8), which encodes a paracrine peptide to delay the onset of pupariation, is controlled by JAK/STAT signaling in early regenerating discs. Our findings suggest that JAK/STAT signaling plays a pivotal role in coordinating regenerative disc growth with organismal developmental timing. In order to analyze transcriptome change in early regenerating imaginal disc, Drosophila prothorasic leg discs were fragmented (to anterior one-quarter or posterior three-quarters) and cultured ex vivo in adult fly abdomen. Regenerating cells in early regeneration phase (at 12 or 24 hours after wounding) were subjected to transcriptome profiling with Affymetrix microarrays. For control samples, the corresponding regions of uncut-cultured discs and uncut-uncultured discs were used.

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Dll and Sp1 ChIP-seq data from Drosophila third instar leg imaginal discs

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