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RNA sequencing to determine gene expression changes in SQLE knockdown osteosarcoma cells

ABSTRACT: RNA sequencing to determine gene expression changes in SQLE knockdown osteosarcoma cells

PROVIDER: PRJNA1028999 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26411255_1.fastq.gz	Fastqsanger.gz
	SRR26411255_2.fastq.gz	Fastqsanger.gz
	SRR26411256_1.fastq.gz	Fastqsanger.gz
	SRR26411256_2.fastq.gz	Fastqsanger.gz
	SRR26411257_1.fastq.gz	Fastqsanger.gz

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RNA sequencing to determine gene expression changes in SQLE knockdown osteosarcoma cells

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p53 transcriptionally regulates SQLE to modulate cholesterol synthesis and tumor growth

Project description:Cholesterol is essential for membrane biogenesis, cell proliferation and differentiation. The role of cholesterol in cancer development and the regulation of cholesterol synthesis are still under active investigation. Here we show that under normal-sterol conditions，p53 directly represses the expression of SQLE, a rate-limiting and the first oxygenation enzyme in cholesterol synthesis，in a SREBP2-independent manner. Through transcriptional downregulation of SQLE, p53 represses cholesterol production in vivo and in vitro, leading to tumor growth suppression. Inhibition of SQLE using small interfering RNA (siRNA) or terbinafine (a SQLE inhibitor) reverses the increased cell proliferation caused by p53 deficiency. Conversely, SQLE overexpression or cholesterol addition promotes cell proliferation, particularly in p53 wild-type cells. More importantly, pharmacological inhibition or shRNA-mediated silencing of SQLE restricts nonalcoholic fatty liver disease (NAFLD) -induced liver tumorigenesis in p53 knockout mice. Therefore, our findings reveal a role for p53 in regulating SQLE and cholesterol biosynthesis, and further demonstrate that downregulation of SQLE is critical for p53-mediated tumor suppression.

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Expression data from human Bladder cancer cell line J82 with control or overexpression of SQLE

Project description:Several reports showed that SQLE was upregulated in some types of cancer, and it is essential for cancer development. Herein, to uncover the role of SQLE in BCa, we performed the RNA-seq to detect the gene expression levels in BCa cell line J82 with overexpression of SQLE.

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