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ABSTRACT: Effect of salivary secretion of Varroa destructor on honeybee

PROVIDER: PRJNA1029743 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26426890_1.fastq.gz	Fastqsanger.gz
	SRR26426890_2.fastq.gz	Fastqsanger.gz
	SRR26426891_1.fastq.gz	Fastqsanger.gz
	SRR26426891_2.fastq.gz	Fastqsanger.gz
	SRR26426892_1.fastq.gz	Fastqsanger.gz

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Effect of Deformed wing virus (DWV) and Varroa destructor (mite) on gene expression in honeybee (Apis mellifra) pupae

Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on global gene expression using microarray transcriptional profiling in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.

2013-06-01 | E-MTAB-1285 | biostudies-arrayexpress

Effects of Deformed wing virus infection and the mite Varroa destructor on siRNA and miRNA composition in the honeybee (Apis mellifera)

Project description:Experiment was designed to study the effect of Deformed wing virus (DWV) and the mite Varroa destructor on on siRNA and miRNA composition using high-throughput sequencing of small RNA in developing worker honeybee (Apis mellifera). Newly hatched bee larvae (day 3 of bee development) were transferred from a Varroa-free colony with low DWV levels to a Varroa-infested colony with high levels of DWV in bees and Varroa mites. All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until their capping (day 8). About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph during pupal development until sampling at purple eye stage (day 14). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in the majority of the mite-exposed pupae compared to the control pupae and the pupae not exposed to Varroa mites.

2013-09-01 | E-MTAB-1671 | biostudies-arrayexpress

Whole genome gene expression differences between a coumaphos resistant and susceptible population of Varroa destructor

Project description:Coumaphos is an acaricide that is widely used to control Varroa mites in bee colonies. However, the extensive use of coumaphos against Varroa has resulted in the development of coumaphos resistant Varroa populations. In this study, the whole genome gene expression profile of 1) a coumaphos resistant V. destructor population (AN-CR) or 2) a coumaphos resistant V. destructor population pretreated with coumaphos (tAN-CR), was compared to the gene expression profile of a susceptible V. destructor population (ATH-S) using Illumina RNAseq.

2021-01-21 | GSE153472 | GEO

Diversity of populations of Deformed wing virus in the honeybee (Apis mellifera) pupae overtly infected by injection of DWV virus preparations from overtly and covertly infected honeybees to haemolymph.

Project description:Experiment was designed (i) to analyse the strain composition of Deformed wing virus (DWV) populations in covertly and overtly infected honeybees (Apis mellifera) from Varroa-free and Varroa-infested colonies, and (ii) to determine abundance of the DWV strains following direct injection of the DWV preparations from covertly and overtly infected bees to the bee pupae haemolymph in the absence of Varroa destructor mites. Experiment included isolation of DWV preparations from the following bees: covertly-infected bees from Varroa-free colony, covertly infected bees exposed orally to the Varroa-selected DWV strains, and the overtly infected Varroa-exposed bees. Honeybee pupae were experimentally injected with those DWV preparations and sampled 4 days post injection following development of overt DWV infection. A series of the DWV cDNA fragment covering complete DWV genomic RNA sequences were amplified by RT-PCR using RNA extracted from virus preparations and the injected pupae. The cDNA preparations were sequenced using next generation(Illumina HighSeq 2000) paired-end sequencing to obtain data on the DWV strain composition.

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Project description:honeybee larval exposure to Varroa destructor triggers specific immune rresponses in different bee castes and species

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Project description:Salivary gland transcriptome of Varroa destructor

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Project description:The tight synchronization between the life cycle of the obligatory parasitic mite Varroa destructor (Varroa) and its host the honeybee, is mediated by honeybee chemical stimuli. These stimuli are mainly perceived by a pit organ located at the distal part of the mite’s foreleg. In the present study, we searched for Varroa chemosensory molecular components by comparing transcriptomic and proteomic profiles between forelegs from different physiological stages, and rear legs. In general, a comparative transcriptomic analysis showed a clear separation of the expression profiles between the rear legs and the three groups of forelegs (phoretic, reproductive and tray-collected mites). Most of the differentially expressed transcripts and proteins in the mite’s foreleg were previously uncharacterized. Using a conserved domain approach, we identified 54 transcripts with known chemosensory-related domains, of which 17 were significantly up regulated in the mite’s forelegs when compared to rear legs. We characterized the chemosensory transcripts using phylogenetic analysis.

2018-11-21 | PXD010042 | Pride

Brain transcriptomics of honey bee parasitized by Varroa destructor or Nosema ceranae

Project description:In this study we addressed whether the transcriptome profile in the honey bee brain is similar for two major parasites of honey bee, Varroa destructor and Nosema ceranae. Honey bees parasitized by these two parasites show accelerated behavioral maturation and deficiences in orientation and learning/memory that we hoped to characterized at the transcriptomic level. honey bee adults infested by Varroa destructor or Nosema ceranae compared to control bees, in duplicate

2013-09-24 | E-GEOD-41109 | biostudies-arrayexpress

Shotgun proteomics of chemosensory organs of Varroa destructor mite

Project description:Aims of the project is the identification of soluble chemosensory proteins in the olfactory organs of the honeybee mite Varroa destructor. Different families of soluble proteins acting as carriers for odorants have been identified in hexapods, while very limited information is available for other terrestrial Arthropoda. In mites and ticks olfactory organs are located on the distal part of the forelegs and on the capitulum appendages. These two body tagmata and the second pair of legs as control, have been dissected out of phoretics and reproductives Varroa destructor females and protein extracts have been analysed through shotgun proteomics. Protein identification and relative quantification (Label-free quantification, LFQ) has been performed using MaxQuant (version 1.5.8.3). Among the proteins more abundant in appendages bearing chemosensory organs, considering as a model hexapoda soluble olfactory proteins, we have searched for small secreted proteins whose structure include a hydrophobic binding pocket.

2018-04-12 | PXD008679 | Pride

Project description:Varroa destructor transcriptome

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