Project description:Parthenogenetic embryos derive their genomes entirely from maternal genome, and lack paternal imprint patterns. Many achievements have been made in the study of genomic imprinting using parthenogenetic embryonic stem cells (hPES cells). However, due to the developmental defects and ethical limits, the comprehensive understanding of parthenogenetic embryo development is still lacking. Here, we induced naive hPES cells into parthenogenic blastoids using a published two-step 3D induction protocol. Morphological and molecular analysis showed that parthenogenetic blastoids contain crucial cell lineages similar to natural human blastocysts.
Project description:ChIP-seq experiment of 14 human lymphoblastoid cell line samples from the 1000 Genomes sample set (http://www.1000genomes.org/). Dataset includes two parent-daughter trios (CEU and YRI populations) and additional eight unrelated individuals (CEU population). This accession contains raw and mapped ChIP-seq read data, other assays in this study are available under accession E-MTAB-1883 (RNA-seq, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1883) and E-MTAB-1885 (GRO-seq, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1885/).
Project description:Transcriptome sequencing (RNA-seq) experiment of 14 human lymphoblastoid cell line samples from the 1000 Genomes sample set (http://www.1000genomes.org/). Dataset includes two parent-daughter trios (CEU and YRI populations) and additional eight unrelated individuals (CEU population). This accession contains raw and mapped RNA-seq read data, other assays in this study are available under accession E-MTAB-1884 (ChIP-seq, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1884) and E-MTAB-1885 (GRO-seq, https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-1885).
