Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of recombinant IL-33s, particularly IL-3395-270, IL-3399-270,IL-33109-270, and IL-33112-270 in HRMVECs

ABSTRACT: Effect of recombinant IL-33s, particularly IL-3395-270, IL-3399-270,IL-33109-270, and IL-33112-270 in HRMVECs

PROVIDER: PRJNA1029963 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effect of recombinant IL-33s, particularly IL-3395-270, IL-3399-270,IL-33109-270, and IL-33112-270 in HRMVECs

Project description:To investigate the expression prolfile of genes regulated by IL-3395-270, IL-3399-270, IL-33109-270, and IL-33112-270 in HRMVECs.

2024-07-24 | GSE245784 | GEO

RNA-seq of mouse primary keratinocytes treated with recombinant IL-33

Project description:Mouse primary keratinocytes were treated with recombinant IL-33 to elucidate the effect of IL-33 signaling on keratinocytes

2021-02-22 | GSE145963 | GEO

Effect of IL-17 on human vascular smooth muscle cells

Project description:Investigate the effect of recombinant human IL-17A on vascular smooth muscle cells cultured from human aortas. Keywords: Dose response

2008-06-28 | GSE11367 | GEO

High salt reduces the activation of IL-4+IL-13 stimulated macrophages

Project description:High salt (NaCl) intake is a major risk factor for cardiovascular disease; high NaCl has also been shown to be a powerful modulator of pro-inflammatory immune cell polarization. To investigate the effect of high NaCl on the polarization of M(IL-4+IL-13) macrophages we performed gene expression analysis by microarray and genome wide histone modification (H3K4me3 and H4ac) analysis by ChIP-seq of murine bone marrow derived macrophages. Macrophages were polarized with recombinant IL-4 and IL-13 in vitro in the presence or absence of an additional 40mM of NaCl in the culture medium for 24h before analysis.

2015-02-07 | E-MTAB-3140 | biostudies-arrayexpress

Effect of recombinant protein CXCL12 on patient-derived glioblastoma stem cells

Project description:To investigate the effect of recombinant CXCL12 on patient-derived glioblastoma stem cells, we treated GSCs with recombinant CXCL12 in cell culture media

2023-12-01 | GSE219288 | GEO

Genome wide identification of BysR binding sites in Burkholderia sp. JP2-270

Project description:To verify genes that are directly regulated by BysR, we used DNA affinity purification sequencing analysis (DAP-seq) for genome-wide recognition of BysR binding sites in vitro. The HALO-fusion BysR protein was successfully expressed and purified. After affinity purification and sequencing, at least 22 million double-end reads per sample were generated and with > 99 % of reads uniquely mapped to the JP2-270 genome. A total of 470 enriched common peaks of two replicates with –log10(P-value) ≥ 2 were called . The mean width of DAP-seq peaks was < 1,000. In total, 367 (78%) of these peaks were found to locate in the -1 kbp to 1 kbp regions by the analysis of peak summit positions relative to the start codons of JP2-270 open reading frames.

2022-11-22 | GSE193916 | GEO

RNA-seq of mouse primary keratinocytes treated with recombinant IL-33

Project description:RNA-seq of mouse primary keratinocytes treated with recombinant IL-33

| PRJNA608891 | ENA

Bradyrhizobium elkanii strain:USDA 270 | isolate:USDA 270

Project description:Bradyrhizobium elkanii USDA 270 genome sequencing

| PRJNA1115718 | ENA

Transcriptional profiling of human IL-9R+ T helper cells in presence of recombinant IL-9 against untreated control

Project description:To study the effect of paracrine interleukin-9 (IL-9) on T helper cells, we incubated IL-9R+ T helper cells isolated from blood (5 samples) and skin (3 samples) with or without IL-9 for 12h and the transcriptome was analyzed by bulk RNA-seq.

2022-10-31 | E-MTAB-12204 | biostudies-arrayexpress

Recombinant GM-CSF enhances the bactericidal ability of PMNs by increasing intracellular IL-1β and improves the prognosis of secondary Pseudomonas aeruginosa pneumonia in sepsis

Project description:This study tested the hypothesis that recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF) enhances polymorphonuclear neutrophils (PMNs) via IL-1β to improve the prognosis of secondary infection in sepsis. The latter stage of sepsis is prone to induce immunosuppression, resulting in secondary fatal infections. rGM-CSF has become a way for sepsis-induced immunosuppression due to its immunomodulatory effect. However, the functional impact of GM-CSF on PMNs in sepsis remains obscure. This study aimed to study the role of rGM-CSF on the bactericidal ability of PMNs in septic mice, assessing its effect on the prognosis of secondary pneumonia, and explore the mechanism of rGM-CSF by intervening PMNs in patients with sepsis. The C57BL/6J sepsis mouse model was induced by cecal ligation and puncture (CLP). rmGM-CSF was used in vivo when mice developed immunosuppression, which was characterized by abnormal bactericidal function of PMNs in peripheral blood. rmGM-CSF improved the prognosis of secondary pneumonia and reversed the function of PMNs. PMNs isolated by Percoll from septic patients were treated by rhGM-CSF in vitro. The expression of CD11b, reactive oxygen species (ROS), phagocytosis and neutrophil extracellular traps (NETs) release in PMNs were enhanced by rhGM-CSF treatments. Whole-transcriptomic sequencing of mouse PMNs indicated that recombinant GM-CSF increased the expression of il1b gene in PMNs. Blocking and inhibiting IL-1β release effectively counteracted the enhancing effect of GM-CSF on the bactericidal function of PMNs. RmGM-CSF enhances the bactericidal function of PMNs in vivo and improves the prognosis of secondary pneumonia in septic mice, and recombinant GM-CSF increases IL-1β precursor reserves, which, if stimulated, can rapidly enhance the bactericidal capacity of PMNs.

2024-03-01 | GSE227162 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data