Project description:Chinese narcissus is well-known monocot plants with beautiful color, fresh and sweet floral scent. A lack of transcriptomic and genomic information hinders our understanding of the molecular mechanisms of narcissus floral scent volatiles biosynthesis. Hence, we hypothesized the functions of the significant differentially expressed genes (DEGs) identified using Illumina RNA-Seq technology, according to public protein annotation databases in this study.
Project description:Gynandropsis gynandra seedlings were germinated then grown in the dark for seven days on 1/2 MS medium, 22oC. Tissue samples were harvested after 0 and 6 hours exposure to WL (100 mol m2 s1) and frozen at -80oC. RNA was prepared from samples using a QIAGEN Plant RNeasy extraction kit and libraries generated using TruSeq RNA Sample v2 kit. Sequencing was performed at the Department of Biochemistry, University of Cambridge using a NextSeq500 Mid Output 150 cycle.
