Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1030932 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparative Transcriptomics Identifies Differentially Expressed Genes Regulating Gametic Development in Arabidopsis thaliana
| PRJNA768783 | ENA
Project description:Geese have a high tolerance of massive energy intake and exhibit little pathological development. We assessed phenotypes and transcriptomes of Tianfu geese to investigate the dynamic expression network behind goose adipogenesis. Goose liver exhibited higher fat accumulation than adipose tissues during fattening. We identified differentially expressed genes that function in several important lipid metabolism pathways, immune response, regulation of cancer, and differentially expressed long noncoding RNAs that might be involved in regulation of these pathways. We found that genes like BGE1 and SCD, which have key roles in glycolysis and synthesis of fatty acids, had higher fold change in liver than in adipose tissue. we suppose that the evolutionary split from mammals in adipogenesis is a result of adaptive evolution to long-distance migration.
2019-01-01 | GSE119421 | GEO
Project description:Purpose: To understand the function differences of goose at broody and breeding stage Methods: RNA-seq analysis of oviduct tissues in reproductive and broody goose Results: Our study screened differential expressed mRNA and pathways involved in broodiness Conclusions:The differential expressed mRNA and pathways identified in this study may contribute to understand the broodiness occurs in goose
2019-04-25 | GSE130245 | GEO
Project description:Lion-head goose is the only large goose species in China, and it was one of the largest goose species in the world. Our previous study firstly reported a chromosome-level genome assembly of Lion-head goose (Anser cygnoides), a native breed in South China, through the combination of PacBio, Bionano, and Hi-C technologies. The fat content of foie gras is augmented during its preparation due to the special feeding regimen. Lion-head geese have a strong tolerance of massive energy intake and show a priority of fat accumulation in liver tissue. In this study, we studied for the first time the important differential genes that regulate fatty liver in Lion-head goose. After high-intake feeding, the fatty livers of Lion-head geese were distinctly characterized. The revelation of gene regulation is an important basis for the study of liver development and molecular characteristics for the Lion-head goose. To analyze the excellent fatty liver performance of Lion-head goose at the molecular level, we performed whole transcriptome analysis by high-throughput RNA sequencing to analyze the key regulatory genes that determine the fatty livers in high-intake feeding group compared with the normal livers in normally-fed Lion-head geese. We identified 716 differentially expressed mRNAs, 145 differentially expressed circRNAs, and 39 differentially expressed lncRNAs in the fatty livers in high-intake feeding group compared with the normal livers in normally-fed Lion-head geese, including upregulated and downregulated genes, respectively. GO enrichment analysis showed that these genes were significantly enriched in molecular function, involved in extracellular regions, DNA-binding transcription factor activity, extracellular matrix, heme binding and other life activities. We chose differentially expressed genes involved in either upregulation or downregulation, and we additionally confirmed the accuracy of sequencing at the RNA level. In summary, our research suggested that these differentially expressed genes may play important roles in fatty liver development in Lion-head goose. However, the functions and mechanisms of these significantly differentially expressed genes should be investigated in future studies.
2023-11-16 | GSE243829 | GEO
Project description:The unique fat storage and metabolic characteristics of goose liver is an important model for studying lipid metabolism in animals or humans. In this study, RNA sequencing technology was used to obtain the liver transcriptome of Sichuan white goose with significant weight difference in the same population, and differentially expressed genes and their pathways were identified, which may help to understand the mechanism of goose weight change. In addition, the identified candidate genes may be useful for molecular breeding of geese.
2020-02-28 | GSE132921 | GEO
Project description:Comparative Transcriptomics Identifies Differentially Expressed Genes Regulating endoreplication and trichome branching
| PRJNA770621 | ENA
Project description:The goals of this project were: to use transcriptomics as a starting point for reverse engineering the NO response network of E. coli, and to identify the targets responsible for NO-induced bacteriostasis. The data is associated with Hyduke DR*, Jarboe LR*, Tran LM, Chou KJY, Liao JC 2007 "Integrated network analysis identifies nitric oxide response networks and dihydroxyacid dehydratase as a crucial target in Escherichia coli. "Proc. Natl. Acad. Sci. USA 104(20):8484-8489. Keywords: Comparative genomic response.
2007-04-26 | GSE7573 | GEO
Project description:The family of apicomplexa-specific proteins with DNA binding AP2 domains (ApiAP2s) includes sequence-specific transcription factors that are key regulators of development in malaria parasites. However, functions for the majority of ApiAP2 genes remain unknown. Here, a systematic knockout screen in Plasmodium berghei identifies ten ApiAP2 genes essential for mosquito transmission, of which four are critical for the formation of infectious ookinetes and three for sporogony. We describe unexpected non-essential functions for AP2-O and AP2-SP proteins in blood stages and identify AP2-G2 as a universal repressor active in both, asexual and sexual stages. Comparative transcriptomics across mutants and developmental stages reveals clusters of co-regulated genes with shared cis elements in their promoters, whose expression can be controlled positively or negatively by different ApiAP2 gene deletions. We propose that stage-specific interactions between ApiAP2 proteins on partly overlapping sets of target genes generate the complex transcriptional network that controls the Plasmodium life cycle.
2017-02-08 | PXD005180 | Pride
Project description:Tumor-associated macrophages (TAM) are a major component of the tumor microenvironment linked to reduced survival in most tumor entities. Using comparative transcriptomics between macrophages derived from spleen and bone marrow at pre-leukemic and leukemic state, we revealed profound re-programming of macrophages during leukemogenesis neither associated with M1-like nor M2-like macrophage programs. Instead, major transcriptional changes were linked to gain of proliferative capacity and loss of phagocytic mechanisms which we experimentally validated in vivo in mice and in vitro in humans. Using Mif-/- mice as a model for reduced tumor burden and prolonged survival of leukemic mice, we demonstrate that transcriptional programs in TAM are further shaped by such environmental factors enhancing tumorigenic effects. Comparative transcriptomics of macrophages within the same tissue at pre-malignant and malignant state lead to the identification of important macrophage-mediated suppressive mechanisms that can form a basis for the development of novel cancer diagnostics and therapeutics.
2017-02-09 | GSE44572 | GEO
Project description:Here we asked whether infiltration of leukemic blasts initiated a response that could be detected in the interstitial fluid phase of the spleen in a rat model known to mimic human acute myeloid leukemia (AML). By cannulating efferent lymphatic vessels, we were able to monitor the response of the spleen microenvironment during leukemia development. Flow cytometric analysis of lymphocytes isolated from spleen lymph showed increased STAT3 and CREB signaling, and proteins related to these pathways were identified with a different profile in leukemic when compared with control spleen lymph. Additionally, SPARC-like 1 protein, recently identified as a promoter of AML cell growth and a biomarker of patient outcome, was locally produced in the spleen and upregulated in the leukemic setting. Thus, interstitial fluid, and its surrogate efferent lymph, can be used to provide unique information about spleen responses and substances released to the general circulation during leukemia development.
2023-06-23 | PXD011399 | Pride