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ABSTRACT:
PROVIDER: PRJNA1032952 | ENA |
REPOSITORIES: ENA
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Project description:The Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characteristics. However, hybrids of Argali sheep and Bashbay sheep are susceptible to Mycoplasma ovipneumoniae infection, the causative agent of mycoplasma ovipneumonia, a chronic respiratory disease that is harmful to the sheep industry. To date, knowledge regarding the mechanisms responsible for M. ovipneumoniae pathogenesis is in scant. Herein, we report the results of transcriptome profiling of lung tissues from Argali hybrid sheep experimentally infected with an M. ovipneumoniae strain at 4 and 14 days post-infection, in comparison to mock-infected animals (0 d). Transcriptome profiling was performed by deep RNA sequencing, using the Illumina platform. The analysis of differentially expressed genes was performed to determine concomitant gene-specific temporal patterns of mRNA expression in the lungs after M. ovipneumoniae infection. We found 156 differentially expressed genes (44 up-regulated, 112 down-regulated) when comparing transcriptomic data at 4 and 0 days post-infection, and 367 (35 up-regulated, 332 down-regulated) when comparing 14 versus 0 days post-infection. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed genes at 4 and 14 versus 0 days post-infection were enriched in 109 and 150 pathways, respectively, and the Primary immunodeficiency pathway was considered most closely related to MO infection (p < 0.01). Hyper-IgM syndrome was identified based on the B-cell immunodeficiency signaling pathway from differentially expressed genes related M. ovipneumoniae infection. Gene Ontology analysis showed that differentially expressed genes in different groups were enriched for 497 and 928 terms, where those most closely related to M. ovipneumoniae infection are ciliated motor damage (p < 0.01). These results could aid in understanding why the Argali hybrid sheep are susceptible to MO as well as how M. ovipneumoniae infection progresses in the lungs and may provide useful information regarding key regulatory pathways.
2019-05-17 | E-MTAB-7936 | biostudies-arrayexpress
Project description:Mycoplasma ovipneumoniae overview
| PRJNA46479 | ENA
Project description:Mycoplasma ovipneumoniae SC01 genome sequencing
| PRJNA63641 | ENA
Project description:Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characters. It is resistant to Mycoplasma ovipneumoniae (MO) infection, the causative agent of Mycoplasma ovipneumonia, a chronic respiratory disease, which is harmful to the sheep industry. As of date, knowledge regarding the mechanisms responsible for MO pathogenesis in scant. Herein, we report the results of transcriptome profiling of the lung tissue of Bashbay sheep experimentally infected with M. ovipneumoniae strain at 4days post-infection(DPI) and 14DPI in comparison to the mock-infected animals (0d), which was performed by Illumina deep sequencing (RNASeq). The analysis of differentially expressed genes (DEGs) was performed to determine the concomitant gene-specific temporal patterns of mRNA expression in the lung after MO infection. The results showed 1048 DEGs (up-regulated: 575; down-regulated: 473) were screened at 4DPI compared to 0d, and 2823 DEGs (up-regulated: 1362; down-regulated: 1461) were screened at 14DPI compared to 0d. KEGG analysis found that the DEGs at 4DPI and 14DPI compared to 0d were enriched in 245 and 287 pathways respectively, the most closely related to MO infection of which was TLR signaling pathway (p<0.01). Two pathways (First: LAMP-TLR2/TLR6-MyD88-MKK6-AP1-IL1B; Second: LAMP-TLR8- MyD88-IRF5-RANTES) were obtained from the figure of TLR signaling pathway that DEGs related with MO infection were mapped to. GO analysis found that the DEGs in different groups were enriched to 1580 and 4561 terms, the most closely releted to MO infection of which was positive regulation of inflammatory response (p<0.01).Our study shouldpave the road for the further analysis of Mycoplasma ovipneumonia.
2018-12-01 | E-MTAB-7408 | biostudies-arrayexpress
Project description:Mycoplasma ovipneumoniae ATCC 29419 Genome sequencing
| PRJNA46459 | ENA
Project description:Mycoplasma ovipneumoniae IK3-3, whole genome shotgun sequencing project
| PRJNA779490 | ENA
Project description:The immune response associated with mastitis caused by Mycoplasma bovis is a very complicated biological process in several type of cells, including immune cells, mammary epithelial cells and, endothelial cells. Thus, revealing of the microRNAs in the Mycoplasma bovis infected mammary gland tissues is particularly important for the immune response mechanism to Mycoplasma bovis. Firstly, mammary gland tissue samples were collected from Holstein cows and screened for Mycoplasma bovis. Then, total RNA was isolated from mycoplasma bovis infected tissues and RNA sequencing was performed. After bioinformatics analysis, GO and KEGG analysis of target genes of identified microRNAs were conducted. Our results revaled that 24 of the known microRNAs were expressed differently and 13 of the novel microRNAs were expressed differently in Mycoplasma bovis positive tissues. The target genes of these microRNAs were found to be associated with especially inflammation pathways. In conclusion, this study demonstrated that identified miRNAs may be involved in the signaling pathways during mastitis case caused by Mycoplasma bovis.
2021-05-25 | GSE152865 | GEO
Project description:We sequenced the miRNAs in the liver tissues of goats to further enrich and elucidate the miRNA expression profile in their physiological cycle. The liver tissues were procured at 5-time points from the Laiwu black goats of 1 day, 2 weeks, 4 weeks, 8 weeks, and 12 weeks of age, respectively with 5 goats per time point, for a total of 25 goats. This study identified 1255 miRNAs.
2021-10-15 | GSE185667 | GEO