Project description:While numerous examples of male reproductive disorders have been reported in vertebrates, invertebrate’s organisms have been considerably less studied, despite their ecological importance. The aim of this study is to investigate male infertility in the amphipod Gammarus fossarum, a sentinel species in freshwater risk assessment. Thus in laboratory, we exposed male gammarids to different concentrations of three different xenobiotics: cadmium, and two potent arthropods endocrine-disruptor chemicals, methoxyfenozide and pyriproxyfen. Afterward, we investigated alterations of reproductive health by sperm quality markers and proteomes dynamics on the male reproductive tissue by nanoLC-MS/MS for evidencing proteins modulated by toxic exposure.
2014-11-04 | PXD001267 | Pride
Project description:The fat body transcriptome of Locusta migratoria under different photoperiod
Project description:The proteomes of three amphipod species, a Holarctic species Gammarus lacustris and two Biakal species Eulimnogammarus verrucosus and Eulimnogammarus cyaneus, were received by LC-MS/MS with TMT-labeling. All animals were collected in August 2018. Before the exposition, animals were acclimated in 6C for 25 days. Three species of amphipods were exposed to heat stress (24.6 C for 24 h) and control temperature (6C for 24 h).
Project description:Transcriptomic data was obtained from adults of the stink bug Nezara viridula to complement biochemical enzymatic activity analysis performed for digestive enzymes. Pooled reads from all sample types were used for de novo assembly of a reference transcriptome. After mapping reads to reference, differential expression was performed between the different tissues of the same diet or between the same tissue in different diets.
Project description:The southeastern Australian epibenthic amphipod Melita plumulosa was exposed to copper via different routes of exposure for 48 hours.
