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Cognate antigen-independent differentiation of resident memory t cells in chronic kidney disease

ABSTRACT: Cognate antigen-independent differentiation of resident memory t cells in chronic kidney disease

PROVIDER: PRJNA1034630 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26632696_1.fastq.gz	Fastqsanger.gz
	SRR26632696_2.fastq.gz	Fastqsanger.gz
	SRR26632697_1.fastq.gz	Fastqsanger.gz
	SRR26632697_2.fastq.gz	Fastqsanger.gz
	SRR26632698_1.fastq.gz	Fastqsanger.gz

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Cognate antigen-independent differentiation of resident memory t cells in chronic kidney disease

Project description:Although resident memory T cells (TRMs), which are memory T cells that are retained locally within tissues, have recently been described as antigen-specific frontline defenders against pathogens in barrier and non-barrier epithelial tissues, they have also been noted for perpetuating chronic inflammation. Furthermore, the conditions responsible for TRM differentiation are still poorly understood, and their contributions, if any, to sterile models of chronic kidney disease (CKD) remain a mystery. In this study we identify a substantial population of TRMs in the kidneys of mice with aristolochic acid (AA)-induced CKD. Flow cytometry of injured kidneys showed T cells bearing TRM surface markers and single cell RNA sequencing revealed these cells as expressing well-known TRM transcription factors and receptors responsible for TRM differentiation and maintenance. While kidney TRMs expressed Cd44, a marker of antigen experience and T cell activation, their derivation was surprisingly independent of cognate antigen-T cell receptor interactions, as the kidneys of transgenic OT-1 mice still harbored considerable proportions of TRMs after injury. Our results suggest a non-antigen-specific or antigen-independent mechanism capable of generating TRMs in the kidney and highlight the need to better understand TRMs and their involvement in CKD.

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Resident memory T cells form during persistent antigen exposure leading to allograft rejection

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Defining DC response to cognate T cell interaction in the antigen-challenged lymph node [dataset 3]

Project description:We designed an experimental set up to probe DC responses to cognate T cell encounter in antigen draining LN

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Development of Fit Epidermal Resident Memory T Cells Requires Antigen Encounter in the Skin

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