Project description:We present a concise workflow to enhance the mass spectrometric detection of cross-linked peptides by introducing sequential digestion and the database search software Xi. We use sequential digestion to reduce the peptide size and increase identification rates. The methodology is independent of samples complexity, cross-linker choice, method acquisition and can be used with multiple digestion steps.
Project description:Anaerobic digestion is a popular and effective microbial process for waste treatment. The performance of anaerobic digestion processes is contingent on the balance of the microbial food web in utilizing various substrates. Recently, co-digestion, i.e., supplementing the primary substrate with an organic-rich co-substrate has been exploited to improve waste treatment efficiency. Yet the potential effects of elevated organic loading on microbial functional gene community remains elusive. In this study, functional gene array (GeoChip 5.0) was used to assess the response of microbial community to the addition of poultry waste in anaerobic digesters treating dairy manure. Consistent with 16S rRNA gene sequences data, GeoChip data showed that microbial community compositions were significantly shifted in favor of copiotrophic populations by co-digestion, as taxa with higher rRNA gene copy number such as Bacilli were enriched. The acetoclastic methanogen Methanosarcina was also enriched, while Methanosaeta was unaltered but more abundant than Methanosarcina throughout the study period. The microbial functional diversity involved in anaerobic digestion were also increased under co-digestion.
Project description:we propose that the clock plays an important role in fine-tuning the hypoxic response under pathophysiological conditions, which can serve as a strategy to reduce the severity of fatal hypoxia-related diseases.
Project description:We propose that the clock plays an important role in fine-tuning the hypoxic response under pathophysiological conditions, which can serve as a strategy to reduce the severity of fatal hypoxia-related diseases.
Project description:We focused on how mica fine particle influences macrophage activities. We analyzed altered transcription in RAW 264.7 cells at 0, 12, and 48 h following the stimulation with 100 M-BM-5g/mL of mica fine particles.
