Project description:Blood was extracted from embryonic hearts at E4 and E6 and non-red blood was separated by density gradient centrifugation Keywords: Cell type comparison
Project description:Blood was extracted from embryonic hearts at E4 and E6 and non-red blood was separated by density gradient centrifugation Experiment Overall Design: There are a total of 9 samples which have one biological replicate each, except negative control E6 embryonic heart (used to screen heart contamination in our blood samples - which were drawn from the heart) which has only one sample.
Project description:Single-nucleus RNA sequencing (snRNA-seq) was used to profile the transcriptome of 8,413 nuclei in chicken adult testis. This dataset includes two samples from two different individuals. This dataset is part of a larger evolutionary study of adult testis at the single-nucleus level (97,521 single-nuclei in total) across mammals including 10 representatives of the three main mammalian lineages: human, chimpanzee, bonobo, gorilla, gibbon, rhesus macaque, marmoset, mouse (placental mammals); grey short-tailed opossum (marsupials); and platypus (egg-laying monotremes). Corresponding data were generated for a bird (red junglefowl, the progenitor of domestic chicken), to be used as an evolutionary outgroup.
Project description:Domesticated animals all show the same patterns regarding phenotypic traits and behaviour, collectively known as the domestic phenotype. All domestic chicken come from the red junglefowl. By keeping three separate populations of junglefowl and selecting for high, low or intermediate fear responses towards humans, the goal is to in the low fear group start to unlock domestic phenotypes.
