Project description:MicroRNA profiling was performed on RNA samples matched to those included in the NIH Human Pluripotent Stem Cell Database (Series GSE32923). Twenty undifferentiated human embryonic stem cell lines and 4 human tissues were analyzed. Expanded descriptions of methods used are available at: http://stemcelldb.nih.gov.
Project description:To better understand the extent to which induced pluripotent stem cells (iPSCs) faithfully recapitulate the characteristics of embryonic stem cells (ESCs) under (undiff)erentiated condition, KSR condition and FBS condition and how both compare to somatic tissues under these conditions, we employed whole-genome transcriptome analysis on all twenty one hESC lines available on the pre-2008 NIH Human Pluripotent Stem Cell Registry, eight human iPSCs derived at NIH by retroviral transduction of human fibroblasts and twenty human somatic tissues. One standard culture protocol was used in conjunction with rigorous quality control. Expanded description of methods used and are available at: http://stemcelldb.nih.gov.