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ABSTRACT: Plaque purified recombinant mumps virus containing HCV glycoproteins

PROVIDER: PRJNA1040024 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26819701_1.fastq.gz	Fastqsanger.gz
	SRR26819701_2.fastq.gz	Fastqsanger.gz
	SRR26819702_1.fastq.gz	Fastqsanger.gz
	SRR26819702_2.fastq.gz	Fastqsanger.gz
	SRR26819703_1.fastq.gz	Fastqsanger.gz

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Project description:Recombinant mumps viruses containing HCV glycoproteins

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growth competition assay of Sendai virus and mumps virus

Project description:We evaluate the individual growth of 8 mutants of Sendai virus in the competitive condition under the cultured cells. We also evalate the growth of 6 mutants of mumps virus in the similar competitive condition.

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Project description:Fowlpox virus (here as FP9, a plaque-purified, high passage-attenuated derivative) effectively suppresses induction of the âinnateâ immune responses, notably the Type I interferon system, of the permissive host (chicken). Despite the extensive usage of fowlpox virus as a recombinant vaccine vector in chickens, its immunomodulatory mechanisms remain largely unknown. In this study, a transcriptomic analysis using the Affymetrix GeneChip chicken genome array was performed at the host gene transcription level at 4, 8, 16 and 24 hours post-infection of mock treated and FP9-infected (MOI=5, 2h) chicken embryo fibroblasts (CEF). The experiment was performed in triplicate with three different batches of CEFs. Because fowlpox virus is capable of expressing antigens in mammalian cells, these studies in chicken cells form a baseline for subsequent study of immunomodulation of mammalian innate immune responses.

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