SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus
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ABSTRACT:
PROVIDER: PRJNA1040164 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR26822805_1.fastq.gz | Fastqsanger.gz | |||
| SRR26822805_2.fastq.gz | Fastqsanger.gz | |||
| SRR26822806_1.fastq.gz | Fastqsanger.gz | |||
| SRR26822806_2.fastq.gz | Fastqsanger.gz | |||
| SRR26822807_1.fastq.gz | Fastqsanger.gz |
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Project description:Aspergillus flavus contaminates crops during preharvest and post-harvest periods and produce carcinogenic mycotoxin aflatoxins posing severe threat to food safety and human health. To identify potential targets to tackle aflatoxin contamination, we have characterized a novel Afper1, sharing sequence identity with the yeast protein per1, regulating cell development and secondary metabolism in A. flavus. Notably, proteomics analysis revealed that the enzymes involved in extracellular hydrolases, conidial development, ER homeostasis, and aflatoxin biosynthesis significantly varied. Unexpectedly, enzymes participated in scavenging ROS including catA, catB, cpeB, and sodM were significantly downregulated and the accumulated ROS and sensitivity to hydrogen peroxide were confirmed by experiments. Surprisingly, Afper1 deletion significantly unregulated heterochromatin protein (HepA) and downregulated GNAT family acetyltransferase involved in heterochromatin formation. Accompanying the accumulated ROS and chromatin remodelling, enzymes participating in aflatoxins, ustiloxin B, and gliotoxin were impaired. These results implied that Afper1 affected chromatin remodelling and disturbed ER homeostasis leading to the accumulation of ROS, and ultimately resulted in growth defect and impaired secondary metabolites biosynthesis.
2022-07-13 | PXD030015 | Pride
Project description:In this study, sntB gene deletion (ΔsntB), complementary (Com-sntB), and HA tag fused to snt2 strain (snt2-HA) strains were constructed by using the homologous recombination method, respectively. It was found in this study that SNTB plays a key role in development, secondary metabolism and pathogenicity of A. flavus. The mechanism was explored by Chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq).
2024-11-13 | GSE247683 | GEO
Project description:The molecular mechanisms underlying aflatoxin production have been well-studied in strains of the fungus Aspergillus flavus (A. flavus) under artificial conditions. However, aflatoxin biosynthesis has rarely been studied in natural isolates of A. flavus strains. In the present study, tandem mass tag (TMT) labeling and high-performance liquid chromatography (HPLC) coupled with tandem-mass spectrometry analysiswere used for proteomic quantification in natural isolates of high- and low-aflatoxin-yield A. flavus strains.
2022-02-17 | PXD027517 | Pride
Project description:Aflatoxins are toxic and carcinogenic secondary metabolites produced by the fungi Aspergillus flavus and A. parasiticus. In order to better understand the molecular mechanisms that regulate aflatoxin production, the biosynthesis of the toxin in A. flavus and A. parasticus grown in yeast extract sucrose media supplemented with 50 mM tryptophan (Trp) were examined. A. flavus grown in the presence of 50 mM tryptophan was found to have significantly reduced aflatoxin B1 and B2 biosynthesis, while A. parasiticus cultures had significantly increased B1 and G1 biosynthesis. Microarray analysis of RNA extracted from fungi grown under these conditions revealed seventy seven genes that are expressed significantly different between A. flavus and A. parasiticus, including the aflatoxin biosynthetic genes aflD (nor-1), aflE (norA), and aflO (omtB). It is clear that the regulatory mechanisms of aflatoxin biosynthesis in response to Trp in A. flavus and A. parasiticus are different. These candidate genes may serve as regulatory factors of aflatoxin biosynthesis. Keywords: Aflatoxin, Aspergillus, flavus, Amnio Acids, Tryptophan
2007-06-07 | GSE6182 | GEO
Project description:Gene expression analysis of A. parasiticus grown under conditions conducive and nonconductive for aflatoxin production was evaluated using glass slide microarrays containing the 753 ESTs. A complex regulatory network governs the biosynthesis of aflatoxin. While several genes involved in aflatoxin production are known, their action alone cannot account for its regulation. Arrays of clones from an Aspergillus flavus cDNA library and glass slide microarrays of ESTs were screened to identify additional genes. An initial screen of the cDNA clone arrays lead to the identification of 753 unique ESTs. Many showed sequence similarity to known metabolic and regulatory genes; however, no function could be ascribed to over 50% of the ESTs. Gene expression analysis of Aspergillus parasiticus grown under conditions conducive and non-conductive for aflatoxin production was evaluated using glass slide microarrays containing the 753 ESTs. Twenty-four genes were more highly expressed during aflatoxin biosynthesis and 18 genes were more highly expressed prior to aflatoxin biosynthesis. No predicted function could be ascribed to 18 of the 24 genes whose elevated expression was associated with aflatoxin biosynthesis. Keywords = Aspergillus Keywords = aflatoxin Keywords: time-course
2003-01-15 | E-GEOD-204 | biostudies-arrayexpress
Project description:purpose: to further gain insight into the effect of RGS proteins on aflatoxin biosynthesis in A. flavus NRRL3357. methods: two mutants ΔrgsA and ΔrgsE separately compared to WT were chose to conduct the analysis of transcriptomics. results: 1750 DEGs are found, including 720 upregulated genes and 1030 downregulated genes in (ΔrgsA vs WT); aflB, aflD, aflF, aflG, aflN, aflP, aflS from ΔrgsE were transcriptionally reduced relative to ΔrgsA in their expressions.
2022-02-21 | GSE196717 | GEO
Project description:Aflatoxins are carcinogenic fungal secondary metabolites. Levels of aflatoxins in agricultural commodities are stringently regulated by many countries. A cluster of genes is responsible for aflatoxin biosynthesis by Aspergillus flavus and other closely related species. Expression of the clustered aflatoxin genes is governed by a complex network of regulatory mechanisms. To better understand the molecular events that are associated with aflatoxin production, transcription profiling by microarray analyses which compared three independent aflatoxigenic A. flavus strains to individual isogenic progenies that no longer produced aflatoxins after serial transfers was carried out. Twenty-two significantly differentially expressed features were identified. After physical mapping using the A. oryzae genome sequence as the reference, the number of unique genes was reduced to 16. Compared to the parental strains, changes in the aflatoxin gene expression levels in the progenies were not significant, which suggests that the inability to produce aflatoxins is not caused by decreased expression. The only gene showing higher expression levels in the progenies is homologous to glutathione S-transferease genes. Overexpression of this gene, named hcc, at six- to nine-fold in an aflatoxigenic A. flavus did not cause discernible changes in colony morphology or aflatoxin production. Loss of aflatoxin production after serial transfers may not result from a single event but caused by multiple factors. Keywords: Compartiave hybridization toxigenic and atoxigenic lines of Aspergillus
2008-06-18 | GSE8185 | GEO
Project description:Aspergillus flavus contaminates crops during preharvest and post-harvest periods and produce carcinogenic mycotoxin aflatoxins posing severe threat to food safety and human health. Lysine 2-hydroxyisobutyrylation is one of the most important reversible post-translational modifications and plays a vital regulatory role in various cellular processes. In order to explore the potential roles of lysine 2-hydroxyisobutyrylation in aflatoxin biosynthesis, protein 2-hydroxyisobutyrylation analysis of A. flavus was performed, and a total of 7156 2-hydroxyisobutyrylation sites in 1473 proteins were identified.
2022-06-06 | PXD030912 | Pride
Project description:Gene expression analysis of A. parasiticus grown under conditions conducive and nonconductive for aflatoxin production was evaluated using glass slide microarrays containing the 753 ESTs. A complex regulatory network governs the biosynthesis of aflatoxin. While several genes involved in aflatoxin production are known, their action alone cannot account for its regulation. Arrays of clones from an Aspergillus flavus cDNA library and glass slide microarrays of ESTs were screened to identify additional genes. An initial screen of the cDNA clone arrays lead to the identification of 753 unique ESTs. Many showed sequence similarity to known metabolic and regulatory genes; however, no function could be ascribed to over 50% of the ESTs. Gene expression analysis of Aspergillus parasiticus grown under conditions conducive and non-conductive for aflatoxin production was evaluated using glass slide microarrays containing the 753 ESTs. Twenty-four genes were more highly expressed during aflatoxin biosynthesis and 18 genes were more highly expressed prior to aflatoxin biosynthesis. No predicted function could be ascribed to 18 of the 24 genes whose elevated expression was associated with aflatoxin biosynthesis. Keywords = Aspergillus Keywords = aflatoxin Keywords: time-course
2003-01-15 | GSE204 | GEO
Project description:Aflatoxins are carcinogenic fungal secondary metabolites. Levels of aflatoxins in agricultural commodities are stringently regulated by many countries. A cluster of genes is responsible for aflatoxin biosynthesis by Aspergillus flavus and other closely related species. Expression of the clustered aflatoxin genes is governed by a complex network of regulatory mechanisms. To better understand the molecular events that are associated with aflatoxin production, transcription profiling by microarray analyses which compared three independent aflatoxigenic A. flavus strains to individual isogenic progenies that no longer produced aflatoxins after serial transfers was carried out. Twenty-two significantly differentially expressed features were identified. After physical mapping using the A. oryzae genome sequence as the reference, the number of unique genes was reduced to 16. Compared to the parental strains, changes in the aflatoxin gene expression levels in the progenies were not significant, which suggests that the inability to produce aflatoxins is not caused by decreased expression. The only gene showing higher expression levels in the progenies is homologous to glutathione S-transferease genes. Overexpression of this gene, named hcc, at six- to nine-fold in an aflatoxigenic A. flavus did not cause discernible changes in colony morphology or aflatoxin production. Loss of aflatoxin production after serial transfers may not result from a single event but caused by multiple factors. Keywords: Compartiave hybridization toxigenic and atoxigenic lines of Aspergillus Aspergillus flavus NRRL 29459, NRRL 29474, and NRRL 29490 are aflatoxigenic strains originated from soil collection in a peanut field (Terrell Co., Georgia, USA). Strains 459B-20-2, 474A-20, and 499A-20 were nonaflatoxigenic isolates obtained after 20 serial transfers of the parental strains on potato dextrose agar slants (Horn and Dorner 2002). Comparsions in each experiment consisted of one aflatoxigenic parental strain and one nonaflatoxigenic progeny, compared after 48- or 72-hr growth. Each comparison was repeated with duplicate dye-flip.
2010-05-26 | E-GEOD-8185 | biostudies-arrayexpress