Project description:Interventions: Nutrition intervention group 1:Perioperative oral general sugar clear liquid diet (200 ml/bag) at time points;Nutrition intervention group 2:Perioperative Oral Complex Carbohydrate Purified Liquid Food (200ml/bag);Blank control group 3:Fasting from food and water 12 hours before surgery and 24 hours after surgery
Primary outcome(s): Intestinal barrier function;Semiquantitative typing of intestinal flora;Gut flora 16sRNA assay;albumin;Length of stay in hospital;Hospitalisation costs
Study Design: Parallel
Project description:As a good niche for bacterial growth, appendix plays a very important role in maintaining and protecting intestinal symbiotic flora. Appendectomy will lead to intestinal microecological disorders. Therefore, we put forward the hypothesis that "appendectomy leads to intestinal microecological disorders and then increases the risk of colorectal cancer". In this study, the feces of normal controls, appendectomy and patients with colorectal cancer were collected, and the specific changes of intestinal flora after appendectomy were explored in detail from the level of family, genus and species by macrogenomic sequencing. Then through functional gene analysis, metabolic pathway analysis and other methods to explore the molecular mechanism of colorectal cancer risk changes and the changes of microflora involved, and verified by mouse fecal bacteria transplantation animal experiment.
Project description:To explore the regulatory mechanism of intestinal flora in Citrobacter rodentium -induced intestinal infection by transcriptome analysis at miRNA molecular level.
Project description:By measuring the intestinal flora abundance and bacterial count of patients in the early stage of using pyrotinib to clarify the relationship between diarrhea caused by pyrotinib and changes in intestinal flora in breast cancer patients, the correlation between the change of intestinal flora and the relief of diarrhea are also explored after two-cycle treatment.
Project description:Interventions: experimental group:Zhenqi Liujun cancer suppression soup and one plus minus zhengqi powder
Primary outcome(s): Intestinal flora 16SRDNA sequencing;Peripheral blood PD-1 and PD-L1
Study Design: Single arm
