Project description:Analysis of the specific transcriptional changes on DCs provided by direct pattern recogition receptor (PRR) or IFNAR signaling that are required for DC maturation after poly IC stimulation. Results provide important information about the intricate differentiation process of DC maturation and the importance of type I IFNs for DC immunogenicity. WT/IFNA-/- or WT/PRR-/- mixed-chimera mice were injected with 50 ug Poly-IC i.p. in vivo 4 and 14 hr later, wild type and KO CD11chi CD3- DX5- B220- DCs were FACS sorted based on CD45.2 expression. Total RNA was isolated and expression profile was compared between unstimulated and activated WT and KO DCs.

2013-10-01 | E-GEOD-46478 | biostudies-arrayexpress

Transcriptional profile of WT, PRR and IFN-/- dendritic cells stimulated with poly IC in vivo

2013-10-01 | GSE46478 | GEO

Human dermal endothelial cells co-treated with dsRNA mimic poly IC and antimicrobial peptide LL-37

Project description:Next generation sequencing (RNA-seq) was used to study the global effects of co-treatment of endothelial cells with poly IC and LL-37. This lead to identification of differentially regulated genes and enriched pathways

2019-04-02 | GSE129152 | GEO

Transcription profiling of primary human astrocytes from brain treated by stathmin, poly IC and ultrapure LPS to examine the effects of different Toll-like receptor agonists

Project description:Goal of the experiment was to examine the effects of different Toll-like receptor agonists on the human astrocyte response, in termsof levels of transcripts encoding cytokines, chemokines and theirreceptors. Stathmin and poly IC are considered as agonist for TLR3, LPSas an agonist for TLR4. The manuscript we are about to submit to Journal of Immunology contains these data, and identifies stathmin, aprotein, as a novel TLR3 agonist. We all know that poly IC is, and bycomparing the transcript responses in astrocytes to either poly IC andstathmin, and observing the similarity in these responses, the experiment adds to the evidence that stathmin indeed activates a verysimilar cellular response as poly IC. The LPS-induced response,mediated by TLR4, is included as a reference, to illustrate thatanother TLR-mediated reaction produces quite something different.

2010-02-11 | E-TABM-851 | biostudies-arrayexpress

NOTCH3-IC

Project description:NOTCH3-IC

| PRJNA480489 | ENA

Peroxidase plays important roles in soybean tolerance to Al toxicity

Project description:Peroxidase plays important roles in soybean tolerance to Al toxicity

| PRJNA480265 | ENA

MtBZR1 plays an important role in nodule development in Medicago truncatula

Project description:MtBZR1 plays an important role in nodule development in Medicago truncatula

| PRJNA545836 | ENA