Project description:This SuperSeries is composed of the following subset Series: GSE32542: Murine serum reactivity to common autoantigens in response to immunization with neutrophil extracellular traps GSE32543: Human and murine serum reactivity to specific histone posttranslational modifications in neutrophil extracellular traps Refer to individual Series
Project description:A microarray analysis was conducted to investigate transcriptional differences between traps and vegetative hyphae of the nematophagous fungus Monacrosporium haptotylum. Our goal was to identify genes that show differential regulation in the two different cell types; 1) traps (knobs) and 2) vegetative hyphae (mycelium) grown in the same medium. The entire design involved nine slides; four of these were hybridisations of knobs versus mycelium and five were mycelium against mycelium, 10 labelled extracts, and 3 biological replicates. The microarray experiments were designed as two-sample comparisons (i.e. knobs versus mycelium or mycelium versus mycelium) using three independent biological replicates, which also included technical and dye-swapped control hybridisations.
