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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [Ribo-seq]

ABSTRACT: Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [Ribo-seq]

PROVIDER: PRJNA1049048 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27103802.fastq.gz	Fastqsanger.gz
	SRR27103803.fastq.gz	Fastqsanger.gz
	SRR27103804.fastq.gz	Fastqsanger.gz
	SRR27103805.fastq.gz	Fastqsanger.gz
	SRR27103806.fastq.gz	Fastqsanger.gz

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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

Project description:Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [RNA-seq]

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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [Ribo-seq]

Project description:Translational control during the intricate process of sporulation in Bacillus subtilis as a response to nutrient limitation is still underexplored. Here, we employed a comprehensive approach including RNA-seq, ribosome profiling and fluorescence microscopy to dissect the translational landscape of B. subtilis during sporulation. We identified two events of translation silencing and described the spatiotemporal changes in the subcellular location of translational machinery during sporulation. Using a triple knock-out strain (3KO) of zinc-independents paralogs of three zinc-dependent ribosomal proteins L31, L33 and S14, we investigated the potential regulatory role of ribosome during sporulation. The 3KO strain exhibited delayed sporulation, reduced germination efficiency, and dysregulated translation including expression of key metabolic and sporulation-related genes as well as disruptions in translation silencing, particularly in late sporulation.

2023-12-06 | GSE249448 | GEO

Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

Project description:The transcriptional control of sporulation in Bacillus subtilis is reasonably well understood, but its translational control is underexplored. Here, we use RNA-seq, ribosome profiling and fluorescence microscopy to study the translational dynamics of B. subtilis sporulation. We identify two events of translation silencing and describe spatiotemporal changes in subcellular localization of ribosomes during sporulation. We investigate the potential regulatory role of ribosomes during sporulation using a strain lacking zinc-independent paralogs of three zinc-dependent ribosomal proteins (L31, L33 and S14). The mutant strain exhibits delayed sporulation, reduced germination efficiency, dysregulated translation of metabolic and sporulation-related genes, and disruptions in translation silencing, particularly in late sporulation. This SuperSeries is composed of the SubSeries listed below.

2023-12-06 | GSE249450 | GEO

Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation [RNA-seq]

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Translation in Bacillus subtilis is spatially and temporally coordinated during sporulation

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DIURNAL RHYTHMS SPATIALLY AND TEMPORALLY ORGANIZE AUTOPHAGY

Project description:RAW spectrum files associated with "DIURNAL RHYTHMS SPATIALLY AND TEMPORALLY ORGANIZE AUTOPHAGY"

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Mapping alterations spatially and temporally during early stages of breast tumourigenesis

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Temporally & Spatially-Resolved Microproteomics and MALDI MSI Lipidomic Analysis for Traumatic Brain Injury

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