Project description:Chinese giant salamander Andrias davidianus has strong tolerance to starvation. Here, a high-throughput proteomic analysis was carried out on liver samples obtained from the A. davidianus after 3, 7 and 11 months of fasting.s a result, the expression levels of 364 proteins were significantly changed in the fasted liver. This study provides insights into the molecular mechanisms underlying the metabolic response of A. davidianus liver to fasting.
Project description:The Chinese giant salamander (CGS, Andrias davidianus) is the largest and longest-lived amphibian. Global and quantitative proteome analysis of multiple tissues would indicate tissue-specific mechanisms and investigate the function of each protein from a whole-organism perspective. Herein, this study performed proteome analysis of eleven tissues collected from adult female CGS using isobaric tags for relative and absolute quantitation (iTRAQ) coupled with LC-MS/MS methods. Based on the predicted protein database from CGS transcriptome database previously obtained, we identified a total of 4607 proteins with quantitative information. Among them, 2153 proteins were shared by two iTRAQ 8-plex experiments with liver as common sample, and these proteins were used for subsequent analysis. Our data will be useful for future research into this large-genome species, as well as for vertebrate-wide comparative genomic, transcriptomic and proteomic studies.